DNA methylation is involved in sexual differentiation and sex chromosome evolution in the dioecious plant garden asparagus.
DNA methylation is involved in sexual differentiation and sex chromosome evolution in the dioecious plant garden asparagus.
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DOI：
10.1038/s41438-021-00633-9
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发表时间：
2021-09-01
影响因子：
8.7
通讯作者：
Gao WJ
中科院分区：
文献类型：
--
作者：
Li SF;Lv CC;Lan LN;Jiang KL;Zhang YL;Li N;Deng CL;Gao WJ
DNA甲基化是许多生物过程中的重要调控机制。然而，有关DNA甲基化对雌雄异株植物性别分化的影响的研究却很少。在这项研究中，我们调查了甲基化和转录模式的差异，男性和女性的花园芦笋花。与雄花相比，同一时期雌花的DNA甲基化水平较高。雄花和雌花从减数分裂前到减数分裂阶段都获得了DNA甲基化。详细的分析显示，DNA甲基化的增加主要是由于CHH甲基化的增加。差异表达基因和差异甲基化区域的相关性分析表明，DNA甲基化可能不是性别决定基因SOFF和TDF1表达变异的原因，但可能在石刁柏性别分化和花发育中起重要作用。上调基因AoMS 1、AoLAP 3、AoAMS和AoLAP 5的CHH区域甲基化程度不同，可能与芦笋的性分化和花发育有关。植物激素信号转导基因和转录因子基因也参与了性分化和花的发育，具有潜在的表观遗传调控作用。此外，CG和CHG甲基化水平在Y染色体上明显高于X染色体，这意味着DNA甲基化可能参与了Y染色体的进化。这些数据提供了深入了解性分化和花发育的表观遗传修饰，并提高了我们对芦笋性染色体进化的理解。
DNA methylation is a crucial regulatory mechanism in many biological processes. However, limited studies have dissected the contribution of DNA methylation to sexual differentiation in dioecious plants. In this study, we investigated the variances in methylation and transcriptional patterns of male and female flowers of garden asparagus. Compared with male flowers, female flowers at the same stages showed higher levels of DNA methylation. Both male and female flowers gained DNA methylation globally from the premeiotic to meiotic stages. Detailed analysis revealed that the increased DNA methylation was largely due to increased CHH methylation. Correlation analysis of differentially expressed genes and differentially methylated regions suggested that DNA methylation might not have contributed to the expression variation of the sex-determining genes SOFF and TDF1 but probably played important roles in sexual differentiation and flower development of garden asparagus. The upregulated genes AoMS1, AoLAP3, AoAMS, and AoLAP5 with varied methylated CHH regions might have been involved in sexual differentiation and flower development of garden asparagus. Plant hormone signaling genes and transcription factor genes also participated in sexual differentiation and flower development with potential epigenetic regulation. In addition, the CG and CHG methylation levels in the Y chromosome were notably higher than those in the X chromosome, implying that DNA methylation might have been involved in Y chromosome evolution. These data provide insights into the epigenetic modification of sexual differentiation and flower development and improve our understanding of sex chromosome evolution in garden asparagus.
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影响因子：
6.9
作者：
Han, Jiangyuan;Hu, Yan;Huang, Dejun
通讯作者：
Huang, Dejun
影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
Sumner, Lloyd W.
影响因子：
12.3
作者：
Akalin A;Kormaksson M;Li S;Garrett-Bakelman FE;Figueroa ME;Melnick A;Mason CE
通讯作者：
Mason CE
影响因子：
4.6
作者：
An YC;Goettel W;Han Q;Bartels A;Liu Z;Xiao W
通讯作者：
Xiao W
DOI：
10.1073/pnas.1815441116
发表时间：
2019-01-22
影响因子：
11.1
作者：
Huang, Huan;Liu, Ruie;Lang, Zhaobo
通讯作者：
Lang, Zhaobo