Glutathione S-transferase P1 correlated with oxidative stress in hepatocellular carcinoma.
Glutathione S-transferase P1 correlated with oxidative stress in hepatocellular carcinoma.
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谷胱甘肽 S-转移酶 P1 与肝细胞癌氧化应激相关
DOI：
10.7150/ijms.5947
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发表时间：
2013
影响因子：
3.6
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Li T;Zhao XP;Wang LY;Gao S;Zhao J;Fan YC;Wang K
背景资料：谷胱甘肽-S-转移酶P1（GSTP 1）是一种重要的II相酶，可以保护各种人类癌症中的细胞免受氧化应激。然而，很少有临床研究进行谷胱甘肽S转移酶1和氧化应激在肝细胞癌（HCC）的关系。因此，本研究旨在评估GST P1和肝癌患者氧化应激之间的潜在关联。研究方法：采用流式细胞术检测38例肝癌患者和38例慢性B型肝炎（CH B）患者外周血单个核细胞（PBMC）中谷胱甘肽S转移酶1（GST 1）的表达。采用实时定量聚合酶链反应（RT-PCR）检测GSTP 1 mRNA水平。采用酶联免疫吸附法（ELISA）检测血浆丙二醛（MDA）、黄嘌呤氧化酶（XOD）、还原型谷胱甘肽（GSH）和谷胱甘肽-S-转移酶（GST）水平。结果：肝细胞癌患者肝组织中GST P1蛋白表达明显低于慢性乙型肝炎患者（P<0.05）。肝细胞癌患者GST P1 mRNA表达较慢性乙型肝炎患者明显降低（P<0.05）。肝细胞癌患者血浆MDA和XOD水平显著高于慢性乙型肝炎患者，而GSH和GST水平显著低于慢性乙型肝炎患者。GSTP 1表达水平与血浆MDA（P<0.01）、XOD（P = 0.01）及GSH（P< 0.01）、GST（P<0.01）水平相关。结论：GSTP 1表达降低可能参与了CHB向HCC发展的氧化应激过程。
Background: Glutathione-S-transferase P1 (GSTP1) is an important phase II enzyme that can protect cells from oxidative stress in various human cancers. However, few clinical studies were undertaken on the relationship between GSTP1 and oxidative stress in hepatocellular carcinoma (HCC). The present study was therefore aimed to evaluate the potential associations between GSTP1 and oxidative stress in HCC patients. Methods: The GSTP1 expression in peripheral blood mononuclear cells (PBMCs) was determined by flow cytometry from 38 HCC patients and 38 chronic hepatitis B (CHB) patients. The GSTP1 mRNA level in PBMCs was determined by real-time quantitative polymerase chain reaction. Enzyme-linked-immunosorbent-assay (ELISA) was performed to measure the oxidative stress status, including plasma levels of malondialdehyde (MDA), xanthine oxidase (XOD), reduced glutathione hormone (GSH) and glutathione-S-transferases (GST). Results: Significantly decreased GSTP1 protein expression was found in HCC patients than in CHB patients (P<0.05). The GSTP1 mRNA expression of HCC patients was also decreased compared with CHB patients (P<0.05). MDA and XOD levels were significantly higher in HCC patients than in CHB patients, while plasma GSH and GST levels were statistically lower in HCC patients than in CHB patients. GSTP1 expression level was correlated with plasma levels of MDA (P<0.01), XOD (P = 0.01) and GSH (P< 0.01), GST (P< 0.01). Conclusion: We demonstrated that the reduced GSTP1 expression might contribute to oxidative stress in the development of HCC from CHB.
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影响因子：
7.4
作者：
Reuter, Simone;Gupta, Subash C.;Chaturvedi, Madan M.;Aggarwal, Bharat B.
通讯作者：
Aggarwal, Bharat B.
影响因子：
7.4
作者：
Marra M;Sordelli IM;Lombardi A;Lamberti M;Tarantino L;Giudice A;Stiuso P;Abbruzzese A;Sperlongano R;Accardo M;Agresti M;Caraglia M;Sperlongano P
通讯作者：
Sperlongano P
影响因子：
11.5
作者：
Lee, JM;Wu, MT;Chen, CJ
通讯作者：
Chen, CJ
影响因子：
6
作者：
Lee, S;Lee, HJ;Kang, GH
通讯作者：
Kang, GH
影响因子：
2.5
作者：
Fujita, N.;Sugimoto, R.;Takei, Y.
通讯作者：
Takei, Y.