CMDH4 encodes a protein that is required for lead tolerance in Arabidopsis
CMDH4 encodes a protein that is required for lead tolerance in Arabidopsis
复制标题
CMDH4 编码拟南芥耐铅所需的蛋白质
DOI：
10.1007/s11104-016-3078-8
复制
发表时间：
2017-03
期刊：
影响因子：
4.9
通讯作者：
Cao Shuqing
中科院分区：
文献类型：
--
作者：
Yang Libo;Fan Tingting;Guan Lingxia;Ren Yongbing;Han Yi;Liu Quoqing;Liu Yongsheng;Cao Shuqing
背景与目的铅（Pb）是对植物和动物具有高毒性的最危险的土壤污染物之一。然而，对铅耐受性的分子机制了解甚少。在这里，我们发现了一个以前未知的细胞质定位的苹果酸脱氢酶（称为CMDH4），它参与调节拟南芥的铅耐受性。方法采用st - dna插入突变体和过表达系进行功能分析。铅是外源供应的，并进行表型分析。结果Pb胁迫诱导scmdh4转录。CMDH4功能缺失导致NAD(P)H水平降低，并对铅胁迫产生超敏反应。进一步分析显示，在cmdh4突变体中，内部H2O2浓度和细胞损伤升高。外源NAD(P)H可以提高植物对Pb胁迫的耐受性，使cmdh4功能缺失突变体的敏感性恢复到野生型水平。此外，编码Pb2+泵的PDR12的转录水平在cmdh4突变体中显著降低，从而导致铅积累增加。综上所述，我们的数据证明了CMDH4是拟南芥耐铅和减少铅积累所必需的。
Background and aimsLead (Pb) is one of the most dangerous soil pollutants with high toxicity to plants and animals. However, the molecular mechanisms behind Pb tolerance are poorly understood. Here we identified a previously unknown cytosol-localized malate dehydrogenase (designated as CMDH4), which is involved in regulating Pb tolerance in Arabidopsis.MethodsT-DNA insertional mutants and overexpression lines were used for functional analysis. Lead was externally supplied and phenotypic analysis of these plants was performed.ResultsCMDH4 transcription was induced by Pb stress. Loss-of-function of CMDH4 resulted in reduced NAD(P)H level, and Pb hypersensitivity in response to Pb stress. Further analysis revealed that internal H2O2 concentration and cellular damage was elevated in cmdh4 mutants. External supply of NAD(P)H could improve plant tolerance to Pb stress, and recover the sensitivity of cmdh4 loss-of-function mutants to the wild-type level. In addition, the transcription level of PDR12 coding for a Pb2+ pump was significantly reduced in cmdh4 mutants, thereby leading to increased Pb accumulation.ConclusionsTo sum up, our data provide evidence that CMDH4 is required for lead tolerance and reduced Pb accumulation in Arabidopsis.
登录
查看更多内容
DOI：
10.1016/b978-0-12-472702-1.50028-1
发表时间：
1975
期刊：
--
影响因子：
--
作者：
Irwin P. Ting;I. Führ;Russell Curry;W. C. Zschoche
通讯作者：
Irwin P. Ting;I. Führ;Russell Curry;W. C. Zschoche
影响因子：
6.9
作者：
Bolwell, GP;Bindschedler, LV;Minibayeva, F
通讯作者：
Minibayeva, F
苹果酸脱氢酶：分布、功能和特性。
影响因子：
1.5
作者：
Rabia Ahmed Musrati;M. Kollarova;N. Mernik;D. Mikulášová
通讯作者：
D. Mikulášová
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan
影响因子：
7.4
作者：
Lee, Sichul;Kim, Yu-Young;An, Gynheung
通讯作者：
An, Gynheung