Genome-wide analysis of MATE transporters and molecular characterization of aluminum resistance in Populus.
Genome-wide analysis of MATE transporters and molecular characterization of aluminum resistance in Populus.
复制标题
MATE 转运蛋白的全基因组分析和杨树铝抗性的分子表征
DOI：
10.1093/jxb/erx370
复制
发表时间：
2017-11-28
影响因子：
6.9
通讯作者：
Luo K
中科院分区：
文献类型：
--
作者：
Li N;Meng H;Xing H;Liang L;Zhao X;Luo K
杨树所有配偶家族成员的鉴定及PtrMATE1介导的杨树根分泌柠檬酸对铝胁迫响应的特征。在占全球耕地约50%的酸性土壤中，离子铝(Al)对大多数植物物种都有很强的毒性。杨树在酸性土壤中自然生长，能耐受高浓度的铝。植物中的多药和有毒化合物挤压(Mate)家族基因参与了植物对铝耐性的反应。然而，到目前为止，交配基因在杨树中的功能作用仍然不清楚。在本研究中，预测了毛果杨基因组中71个可能的配偶转运蛋白。染色体分布、系统发育关系和表达水平分析表明，属于亚组IIIc的4个候选交配基因可能与杨树的高耐铝性有关。此外，铝胁迫还诱导了IIIc亚群成员PtrMATE1和PtrMATE2的表达水平。在洋葱表皮细胞中的瞬时表达表明，PtrMATE1定位于细胞膜。PtrMATE1的过表达增加了铝诱导的转基因植株根尖柠檬酸的分泌。与野生型植物相比，铝诱导的根生长抑制在杨树和拟南芥的PtrMATE1高表达系中都得到了缓解。此外，PtrMATE1在铝胁迫后12h被诱导表达，而PtrMATE2在铝胁迫后24h被诱导表达，这表明这两种蛋白在铝胁迫下协同作用。综上所述，这些结果为揭示杨树耐铝性的分子机制提供了重要的线索。
Identification of all MATE family members in Populus and characterization of PtrMATE1 mediated citrate exudation from the roots of Populus in response to Al stress. Ionic aluminum (Al) in acidic soils, comprising approximately 50% of arable land globally, is highly toxic to most plant species. Populus grow naturally in acidic soils and tolerate high concentrations of Al. Multidrug and toxic compound extrusion (MATE) family genes in plants are involved in responses to Al tolerance. To date, however, the functional roles of the MATE genes in Populus remain unclear. In the present study, 71 putative MATE transporters were predicted in the genome of Populus trichocarpa. The chromosome distribution, phylogenetic relationships, and expression level analysis revealed that four candidate MATE genes belonging to subgroup IIIc might contribute to high Al tolerance in poplar. Further, the expression levels of two subgroup IIIc members, PtrMATE1 and PtrMATE2, were induced by Al stress. Transient expression in onion epidermal cells showed that PtrMATE1 was localized to the plasma membrane. Overexpression of PtrMATE1 increased Al-induced secretion of citrate from the root apex of transgenic plants. Al-induced inhibition of root growths were alleviated in both PtrMATE1 overexpression lines in Populus and in Arabidopsis compared with wild-type plants. In addition, PtrMATE1 expression was induced at 12 h after exposure to Al stress whereas PtrMATE2 expression was induced at 24 h, indicating that these proteins coordinately function in response to Al stress in poplar. Taken together, these results provide important insights into the molecular mechanisms involved in Al tolerance in poplar.
登录
查看更多内容
影响因子：
7.4
作者：
DELHAIZE, E;CRAIG, S;RANDALL, PJ
通讯作者：
RANDALL, PJ
影响因子：
7.4
作者：
DELHAIZE, E;RYAN, PR
通讯作者：
RYAN, PR
影响因子：
2.3
作者：
GORANSSON, A;ELDHUSET, TD
通讯作者：
ELDHUSET, TD
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
毛果杨NAC结构域转录因子基因家族的综合分析
影响因子：
5.3
作者：
Hu R;Qi G;Kong Y;Kong D;Gao Q;Zhou G
通讯作者：
Zhou G