In vivo effects of murine hybridoma monoclonal antibody in a patient with T-cell leukemia.
In vivo effects of murine hybridoma monoclonal antibody in a patient with T-cell leukemia.
复制标题
鼠杂交瘤单克隆抗体对 T 细胞白血病患者的体内作用。
作者：
R. Miller;D. Maloney;J. Mckillop;R. Levy
针对正常T细胞分化抗原的鼠单克隆抗体给予成人T细胞白血病患者。免疫荧光染色显示患者白血病细胞上这种抗原的数量增加。使用竞争性放射免疫测定法，治疗前血清中未检测到游离抗原。使用1 mg剂量进行两个疗程的体内治疗。每种方法都使WBC迅速急剧下降，并在随后的24小时内恢复到治疗前水平，其模式与白细胞去除术相似。首次抗体给药后，血清中可检测到循环游离抗原，并观察到肌酐清除率一过性下降。当时给予5毫克剂量的抗体无效，可能是因为它被游离抗原阻断了。白血病细胞的抗原调节在每个抗体疗程后被发现是短暂的。首次给药后5天，发现宿主抗小鼠抗体应答较弱且临床上不显著。患者耐受抗体治疗没有困难。单克隆抗体作为癌症的免疫治疗方法提供了希望，但这里遇到的问题必须得到解决。
A murine monoclonal antibody directed against a normal T-cell differentiation antigen was given to a patient with adult T-cell leukemia. Immunofluorescence staining showed increased amounts of this antigen on the patient's leukemia cells. Using a competition radioimmunoassay, free antigen was not detectable in the serum prior to therapy. Two courses of in vivo therapy were given using a 1-mg dose. Each produced a prompt and dramatic fall in WBC with return to pretreatment levels over the ensuing 24 hr, a pattern similar to that seen with leukopheresis. After the first dose of antibody, circulating free antigen became detectable in the serum and a transient decline in creatinine clearance was noted. A 5-mg dose of antibody given at that time was ineffective, presumably because it was blocked by free antigen. Antigenic modulation by leukemia cells was found transiently following each course of antibody. A weak and clinically insignificant host antimouse antibody response was found 5 days after the first treatment. The patient tolerated antibody therapy without difficulty. Monoclonal antibodies offer promise as an immunotherapeutic approach to cancer but problems encountered here must be addressed.
研究单克隆抗体识别的人类 T 淋巴细胞抗原。
DOI：
10.1073/pnas.78.3.1791
发表时间：
1981
影响因子：
11.1
作者：
Engleman,EG;Warnke,R;Fox,RI;Dilley,J;Benike,CJ;Levy,R
通讯作者：
Levy,R
影响因子：
5
作者：
Harmsen, M. M.;van Solt, C. B.;van Zijderveld, F. G.
通讯作者：
van Zijderveld, F. G.
单克隆抗体体外诱导人急性淋巴细胞白血病抗原的调节。
DOI：
--
发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ritz,J;Pesando,JM;Notis-McConarty,J;Schlossman,SF
通讯作者：
Schlossman,SF