Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection.
Self-aligned sequential lateral field non-uniformities over channel depth for high throughput dielectrophoretic cell deflection.
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DOI：
10.1039/d0lc01211d
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发表时间：
2021-03-09
期刊：
影响因子：
6.1
通讯作者：
Swami NS
中科院分区：
文献类型：
--
作者：
Huang X;Torres-Castro K;Varhue W;Salahi A;Rasin A;Honrado C;Brown A;Guler J;Swami NS
介电电泳（DEP）通过控制所施加的场的频率，使得能够基于细微的亚细胞表型差异来分离细胞。然而，目前基于电极的几何形状在样品通道的有限深度上延伸，从而降低了操作样品的通量（低于μL/min的流速和<105个细胞/mL）。我们提出了一种流通装置，其具有横向延伸穿过样品通道宽度（100 μm）的自对准顺序场非均匀性，该样品通道宽度由使用单个光刻步骤在样品通道侧壁的整个深度（50 μm）上图案化的金属产生。这使得单细胞流线能够进行渐进的DEP偏转，而对细胞起始位置、其取向与场和细胞间相互作用的依赖性最小。使用健康和去谷胱甘肽固定的红细胞的异质样品验证了表型特异性细胞分离（>μL/min流速和>106个细胞/mL），单细胞阻抗细胞术显示DEP收集的级分是完整的，并显示出与其基于电容的DEP交叉频率一致的电不透明度差异。这种几何形状可以解决用于量化细胞系统的表型异质性的“全电”选择性细胞分离和细胞计数系统的愿景。
Dielectrophoresis (DEP) enables the separation of cells based on subtle subcellular phenotypic differences by controlling frequency of the applied field. However, current electrode-based geometries extend over a limited depth of the sample channel, thereby reducing the throughput of the manipulated sample (sub-μL/min flow rates and <105 cells/mL). We present a flow through device with self-aligned sequential field non-uniformities extending laterally across the sample channel width (100 μm) that is created by metal patterned over the entire depth (50 μm) of the sample channel sidewall using a single lithography step. This enables single-cell streamlines to undergo progressive DEP deflection with minimal dependence on the cell starting position, its orientation versus the field and intercellular interactions. Phenotype-specific cell separation is validated (>μL/min flow and >106 cells/mL) using heterogeneous samples of healthy and gluraldehyde-fixed red blood cells, with single-cell impedance cytometry showing that the DEP collected fractions are intact and exhibit electrical opacity differences consistent with their capacitance-based DEP crossover frequency. This geometry can address the vision of an “all electric” selective cell isolation and cytometry system for quantifying phenotypic heterogeneity of cellular systems.
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影响因子：
2.9
作者：
Lewpiriyawong, Nuttawut;Yang, Chun;Lam, Yee Cheong
通讯作者：
Lam, Yee Cheong
影响因子：
3.7
作者：
Cheung, K;Gawad, S;Renaud, P
通讯作者：
Renaud, P
影响因子：
5
作者：
Cros, Jerome;Raffenne, Jerome;Pote, Nicolas
通讯作者：
Pote, Nicolas
影响因子：
7.4
作者：
Lewpiriyawong, Nuttawut;Kandaswamy, Kumaravel;Stocker, Roman
通讯作者：
Stocker, Roman
通过电介型分离循环肿瘤细胞。
影响因子：
5.2
作者：
Gascoyne PR;Shim S
通讯作者：
Shim S