Neutropenic responses to intradermal injections of Escherichia coli. Effects on the kinetics of polymorphonuclear leukocyte emigration.
Neutropenic responses to intradermal injections of Escherichia coli. Effects on the kinetics of polymorphonuclear leukocyte emigration.
复制标题
皮内注射大肠杆菌的中性粒细胞减少反应。
作者：
M. Cybulsky;I. Cybulsky;H. Movat
将灭活的大肠杆菌注射到兔子体内会引起明显的中性粒细胞减少症，并引起体温迅速上升。中性粒细胞减少的程度和持续时间都是剂量依赖性的。在从中性粒细胞减少症中恢复后，当随后给予等量的大肠杆菌时，兔子对其再发育变得难以接受。检测中性粒细胞减少和随后的不应期对中性粒细胞(PMN)向炎症部位迁移率的影响。将灭活的大肠杆菌(每个部位6×10(8))注射到两组兔的皮内，每组20个部位。第一组(F组)比第二组(S组)早6小时。用~(51)Cr标记细胞定量的PMN迁移动力学在两组中有所不同。在S组，中性粒细胞在0-4h内大量涌入，随后迁出的程度迅速下降。在F组，在最初的4小时内检测到一分钟的PMN流入，与明显的中性粒细胞减少相吻合。测定F组中性粒细胞最大内流时间为6~10小时。4h光镜下可见F组真皮内有少量中性粒细胞浸润，真皮内细菌较多，而S组真皮内有大量细菌吞噬。通过比较两组4h后细菌吞噬的程度和PMN在6~10h内的迁移量，我们得出结论：吞噬灭活的大肠杆菌不是调节PMN局部迁出的主要机制。相反，我们认为，对从大肠杆菌释放的炎性因子的快速止痛或部位脱敏是负责的机制。
Killed Escherichia coli organisms injected intradermally into rabbits induced significant neutropenia and provoked a rapid rise in body temperature. Both the magnitude and the duration of the neutropenia were dose-dependent. After recovery from neutropenia, the rabbits became refractory to its redevelopment when subsequently given an equivalent dose of E coli. The influence of neutropenia and the subsequent refractory period on the rate of polymorphonuclear leukocyte (PMN) emigration into inflammatory sites was examined. Killed E coli organisms (6 X 10(8) per site) were injected into two groups of 20 intradermal sites in each rabbit. The first group (Group F) preceded the second (Group S) by 6 hours. The kinetics of PMN emigration, quantitated with 51Cr-labeled cells, differed in the two groups. In Group S sites an intense PMN influx was measured at 0-4 hours, and subsequently the extent of PMN emigration rapidly declined. In Group F sites a minute PMN influx was detected during the first 4 hours, coinciding with a marked neutropenia. The maximal PMN influx into Group F sites was measured between 6 and 10 hours. Microscopic sections at 4 hours showed a scanty PMN infiltrate and numerous bacteria in the dermis of Group F sites, while extensive phagocytosis of bacteria by PMNs was apparent in Group S sites. By comparing the extent of bacterial phagocytosis in 4-hour-old sites with the magnitudes of PMN emigration between 6 and 10 hours in both groups, we concluded that the phagocytic elimination of killed E coli was not a major mechanism regulating the cessation of local PMN emigration. Instead, we propose that tachyphylaxis or desensitization of sites to inflammatory factors released from E coli is the responsible mechanism.
炎症介质刺激粒细胞粘附于培养的人内皮细胞。
影响因子：
7.5
作者：
Zimmerman,GA;Hill,HR
通讯作者：
Hill,HR
影响因子：
15.9
作者：
BEVILACQUA, MP;POBER, JS;GIMBRONE, MA
通讯作者：
GIMBRONE, MA
DOI：
10.1073/pnas.81.7.2191
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HOOVER, RL;KARNOVSKY, MJ;LEWIS, RA
通讯作者：
LEWIS, RA