Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice.
Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice.
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DOI：
10.3390/v13050728
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发表时间：
2021-04-22
期刊：
影响因子：
--
通讯作者：
Rosenberg HF
中科院分区：
文献类型：
--
作者：
Limkar AR;Percopo CM;Redes JL;Druey KM;Rosenberg HF
呼吸道病毒感染可对肺功能产生长期影响，甚至在急性反应消退后仍持续存在。许多研究已经将儿童早期严重感染呼吸道合胞病毒（RSV）与喘息和哮喘的发展联系起来，尽管这些观察结果之间的潜在机制尚不清楚。在这里，我们研究了野生型小鼠在有症状但亚致死的感染小鼠肺炎病毒（PVM）后恢复后出现的气道高反应性（AHR）。我们发现BALB/c小鼠对有限剂量的PVM有反应，伴随病毒复制、急性炎症和气道中性粒细胞募集的显著但可逆的体重减轻。接种后第21天，气道内不再检测到病毒，急性炎症反应基本消退。然而，与大多数使用PVM感染模型的早期研究相反，所有小鼠都在初始感染中存活，并且都继续产生血清抗PVM IgG抗体。此外，通过有创容积脉搏波描记和精确肺切片，我们发现这些小鼠在接种后第21天表现出明显的气道高反应性，并持续到第45天。总之，我们的发现扩展了一个重要的和通用的呼吸道病毒感染模型，现在可以用来探索病毒粒子和病毒粒子清除以及病毒诱导的炎症介质及其信号通路在病毒后AHR和肺功能障碍的发展和持续中的作用。
Respiratory virus infections can have long-term effects on lung function that persist even after the acute responses have resolved. Numerous studies have linked severe early childhood infection with respiratory syncytial virus (RSV) to the development of wheezing and asthma, although the underlying mechanisms connecting these observations remain unclear. Here, we examine airway hyperresponsiveness (AHR) that develops in wild-type mice after recovery from symptomatic but sublethal infection with the natural rodent pathogen, pneumonia virus of mice (PVM). We found that BALB/c mice respond to a limited inoculum of PVM with significant but reversible weight loss accompanied by virus replication, acute inflammation, and neutrophil recruitment to the airways. At day 21 post-inoculation, virus was no longer detected in the airways and the acute inflammatory response had largely resolved. However, and in contrast to most earlier studies using the PVM infection model, all mice survived the initial infection and all went on to develop serum anti-PVM IgG antibodies. Furthermore, using both invasive plethysmography and precision-cut lung slices, we found that these mice exhibited significant airway hyperresponsiveness at day 21 post-inoculation that persisted through day 45. Taken together, our findings extend an important and versatile respiratory virus infection model that can now be used to explore the role of virions and virion clearance as well as virus-induced inflammatory mediators and their signaling pathways in the development and persistence of post-viral AHR and lung dysfunction.
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DOI：
10.3390/v13010002
发表时间：
2020-12-22
期刊：
Viruses
影响因子：
--
作者：
Mai E;Percopo CM;Limkar AR;Sek AC;Ma M;Rosenberg HF
通讯作者：
Rosenberg HF
DOI：
10.1086/592714
发表时间：
2008-11-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Estripeaut D;Torres JP;Somers CS;Tagliabue C;Khokhar S;Bhoj VG;Grube SM;Wozniakowski A;Gomez AM;Ramilo O;Jafri HS;Mejias A
通讯作者：
Mejias A
影响因子：
13.8
作者：
Bellan M;Soddu D;Balbo PE;Baricich A;Zeppegno P;Avanzi GC;Baldon G;Bartolomei G;Battaglia M;Battistini S;Binda V;Borg M;Cantaluppi V;Castello LM;Clivati E;Cisari C;Costanzo M;Croce A;Cuneo D;De Benedittis C;De Vecchi S;Feggi A;Gai M;Gambaro E;Gattoni E;Gramaglia C;Grisafi L;Guerriero C;Hayden E;Jona A;Invernizzi M;Lorenzini L;Loreti L;Martelli M;Marzullo P;Matino E;Panero A;Parachini E;Patrucco F;Patti G;Pirovano A;Prosperini P;Quaglino R;Rigamonti C;Sainaghi PP;Vecchi C;Zecca E;Pirisi M
通讯作者：
Pirisi M
影响因子：
9.6
作者：
Ong, KC;Ng, AWK;Earnest, A
通讯作者：
Earnest, A
DOI：
10.1152/ajplung.00467.2007
发表时间：
2009-01-01
影响因子：
4.9
作者：
Bem, Reinout A.;van Woensel, Job B. M.;Matute-Bello, Gustavo
通讯作者：
Matute-Bello, Gustavo