Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
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DOI：
10.1016/j.micinf.2005.02.006
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发表时间：
2005-05
影响因子：
5.8
通讯作者：
Yang R
中科院分区：
文献类型：
--
作者：
Qiu M;Shi Y;Guo Z;Chen Z;He R;Chen R;Zhou D;Dai E;Wang X;Si B;Song Y;Li J;Yang L;Wang J;Wang H;Pang X;Zhai J;Du Z;Liu Y;Zhang Y;Li L;Wang J;Sun B;Yang R
一种新型冠状病毒，即严重急性呼吸综合征（SARS）冠状病毒（SARS-CoV），被确定为 SARS 的病原体。针对病毒个体蛋白的特异性抗体谱对于疫苗和诊断工具的开发至关重要。在本研究中，制备了与 SARS-CoV 的 4 个结构蛋白（S、E、M 和 N）和 5 个假定的未表征蛋白（3a、3b、6、7a 和 9b）相关的 13 种重组蛋白，并用于通过蛋白微阵列筛选和监测 SARS 患者血清中的特异性 IgG 抗体。在恢复期SARS患者的血清中检测到了S、3a、N和9b蛋白的抗体，而未检测到E、M、3b、6和7a蛋白的抗体。在可检测的特异性抗体中，抗S和抗N占主导地位，并且可以在SARS患者的血清中持续存在到30周。在针对重组蛋白S3、N、3a和9b的兔抗血清中，只有抗S3血清对Vero E6细胞中的SARS-CoV感染表现出显着的中和活性。结果表明（1）抗S和抗N抗体是诊断标志物，特别是S3具有免疫原性，因此是作为亚单位疫苗抗原的良好候选者； (2)从病毒结构的角度来看，一些人血清中存在与两种重组多肽3a和9b反应的抗体，支持了它们在病毒周期中合成的假设。
A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.
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影响因子：
9.4
作者：
Woo, PCY;Lau, SKP;Yuen, K
通讯作者：
Yuen, K
影响因子：
3.8
作者：
Hai, P;Liu, YG;Rao, ZH
通讯作者：
Rao, ZH
DOI：
10.1074/mcp.m300048-mcp200
发表时间：
2003-05
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Krokhin O;Li Y;Andonov A;Feldmann H;Flick R;Jones S;Stroeher U;Bastien N;Dasuri KV;Cheng K;Simonsen JN;Perreault H;Wilkins J;Ens W;Plummer F;Standing KG
通讯作者：
Standing KG
影响因子：
3.5
作者：
Yu CJ;Chen YC;Hsiao CH;Kuo TC;Chang SC;Lu CY;Wei WC;Lee CH;Huang LM;Chang MF;Ho HN;Lee FJ
通讯作者：
Lee FJ
影响因子：
--
作者：
Qin, E;Zhu, QY;Yang, HM
通讯作者：
Yang, HM