Gaussia luciferase variant for high-throughput functional screening applications.
Gaussia luciferase variant for high-throughput functional screening applications.
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DOI：
10.1021/ac901234r
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发表时间：
2009-08-15
影响因子：
7.4
通讯作者：
Tannous, Bakhos A.
中科院分区：
文献类型：
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作者：
Maguire, Casey A.;Deliolanis, Nikolaos C.;Pike, Lisa;Niers, Johanna M.;Tjon-Kon-Fat, Lee-Ann;Sena-Esteves, Miguel;Tannous, Bakhos A.
Gaussia luciferase（Gluc）是研究基因表达、启动子活性、蛋白-蛋白相互作用、信号转导以及肿瘤细胞生长和治疗反应等不同生物学过程的敏感报告基因。由于Gluc是天然分泌的，这些过程的动力学可以通过在不同时间点测量培养物中条件培养基的等分试样或体内几微升血液来实时监测。Gluc催化具有短半衰期的光发射，这对于某些应用是不利的。我们分离了一种Gluc突变体，其在去污剂存在下催化增强的光稳定性，并具有高灵敏度，使其成为高通量功能筛选应用的有吸引力的荧光素酶。
Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interaction, signal transduction as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.
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影响因子：
3.7
作者：
Badr CE;Hewett JW;Breakefield XO;Tannous BA
通讯作者：
Tannous BA
DOI：
10.1073/pnas.0701185104
发表时间：
2007-04-24
影响因子：
11.1
作者：
Hewett, Jeffrey W.;Tannous, Bakhos;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.
影响因子：
3.5
作者：
Suzuki, Takahiro;Usuda, Shinsuke;Inouye, Satoshi
通讯作者：
Inouye, Satoshi
影响因子：
48
作者：
Remy, Ingrid;Michnick, Stephen W.
通讯作者：
Michnick, Stephen W.
影响因子：
82.9
作者：
通讯作者：
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