Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
复制标题
乳腺过程中乳腺的转录变化显示，通过人牛奶的细胞单细胞测序揭示了。
DOI：
10.1038/s41467-021-27895-0
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发表时间：
2022-01-28
影响因子：
16.6
通讯作者：
Khaled WT
中科院分区：
文献类型：
--
作者：
Twigger AJ;Engelbrecht LK;Bach K;Schultz-Pernice I;Pensa S;Stenning J;Petricca S;Scheel CH;Khaled WT
在正常情况下，成年乳腺最显著的扩张和分化发生在怀孕和哺乳期间对全身生殖激素的反应中，以使乳汁合成和分泌以维持后代。然而，由于获取样本的挑战，在怀孕和哺乳期间发生的人类乳腺组织重塑仍然知之甚少。我们在此报告了110,744个活的乳腺细胞的单细胞转录组学分析，分别从9个和7个供体中分离出人乳或非泌乳乳腺组织。我们发现人乳主要含有属于管腔谱系的上皮细胞和一系列免疫细胞。进一步的乳细胞转录组学分析确定了两种不同的分泌细胞类型，它们与腔祖细胞有相似之处，但没有可与激素反应细胞相比的群体。综上所述，我们的数据提供了一个参考图，并为人类哺乳期间发生的细胞动力学提供了一个窗口，并可能为怀孕、哺乳和乳腺癌之间的相互作用提供进一步的见解。在怀孕和哺乳期间发生的人类乳腺组织重塑仍然知之甚少。在这里，作者描述了人乳中的细胞，鉴定了类似于腔祖细胞和免疫细胞的上皮细胞，为这一过程提供了见解。
Under normal conditions, the most significant expansion and differentiation of the adult mammary gland occurs in response to systemic reproductive hormones during pregnancy and lactation to enable milk synthesis and secretion to sustain the offspring. However, human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood due to the challenge of acquiring samples. We report here single-cell transcriptomic analysis of 110,744 viable breast cells isolated from human milk or non-lactating breast tissue, isolated from nine and seven donors, respectively. We found that human milk largely contains epithelial cells belonging to the luminal lineage and a repertoire of immune cells. Further transcriptomic analysis of the milk cells identified two distinct secretory cell types that shared similarities with luminal progenitors, but no populations comparable to hormone-responsive cells. Taken together, our data offers a reference map and a window into the cellular dynamics that occur during human lactation and may provide further insights on the interplay between pregnancy, lactation and breast cancer. Human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood. Here the authors characterize cells in human milk, identifying epithelial cells resembling luminal progenitors and immune cells, contributing insights into this process.
影响因子：
2.5
作者：
van Amerongen R
通讯作者：
van Amerongen R