Behind the Scenes of the Human Breast Cell Atlas Project.
Behind the Scenes of the Human Breast Cell Atlas Project.
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DOI：
10.1007/s10911-021-09482-7
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发表时间：
2021-03
影响因子：
2.5
通讯作者：
van Amerongen R
中科院分区：
文献类型：
--
作者：
van Amerongen R
随着人类基因组计划的完成[1，2]，以及识别我们DNA中所有功能元件的任务交给了ENCODE联盟[3]，科学家们一定觉得他们已经准备好迎接下一个巨大的挑战：起草人体的大宪章-并且具有单细胞分辨率。这正是人类细胞图谱项目的目标。由美国的Chan Zuckerberg Initiative（CZI），欧盟的Horizon 2020和英国的Wellcome支持的国际联盟努力的总体目标是生成一系列“细胞参考图”，这不仅有助于对人体中存在的数千种不同细胞类型进行编目，而且还应该揭示细胞状态转换，分化轨迹和谱系关系。我承认，作为一个没有直接参与这项工作的人，我并不清楚事情是如何进展的。特别是，我发现很难正确了解人类乳腺细胞图谱的状况，因为稀缺的信息似乎仅限于CZI网站。为了提高该项目的知名度和兴趣，这可能是我们领域的游戏规则改变者，因此我决定利用这期《乳腺生物学和肿瘤杂志》特刊作为联系参与这些开创性阶段的一些主要科学家的借口。幸运的是，他们非常愿意让我了解人类乳腺细胞图谱的过去、现在和未来。继2016年10月在伦敦的启动会议上推出人类细胞图谱（HCA）后，一份白色文件阐述了其目标和范围，强调了实验和计算方法标准化的必要性[4]。众所周知，乳房在创始财团提出的最初生物系统清单中缺席（就像其他生殖组织一样），但几个月后，当Kai Kessenbrock（加州大学欧文分校），德文郡劳森（加州大学欧文分校）和尼古拉斯·纳文（Nicholas Navin）（德克萨斯大学医学博士安德森癌症中心）于2017年2月在斯坦福大学举行的HCA大会上会面。在这里，CZI介绍了该项目及其作为科学家主导的会议的组织-
With the human genome project out of the way [1, 2] and the task of identifying all functional elements in our DNA bestowed upon the ENCODE consortium [3], scientists must have felt they were ready for the next big challenge: that of drafting a magna carta of the human body–and with single cell resolution at that. This is precisely what the Human Cell Atlas project aims to do. The overarching goal of the international consortium effort, backed by the Chan Zuckerberg Initiative (CZI) in the US, Horizon2020 in the EU and Wellcome in the UK, is to generate a collection of “cellular reference maps”, which will not only serve to catalogue the thousands of different cell types present in the human body, but which should also reveal cell state transitions, differentiation trajectories and lineage relationships. I will confess that as someone not (yet) directly involved in the effort, it was not all that clear to me how things were progressing. In particular, I found it difficult to get a proper idea of the status of the Human Breast Cell Atlas, with scarce information seemingly limited to the CZI website. To increase visibility and interest in the project, which could be a game changer in our field, I therefore decided to use this special issue of the Journal of Mammary Gland Biology and Neoplasia as an excuse to contact some of the lead scientists involved in these pioneering stages. Luckily, they were more than willing to give me a behind the scenes glimpse of the past, present and future of the Human Breast Cell Atlas. Following the launch of the Human Cell Atlas (HCA) at a kick-off meeting in London in October 2016, a white paper laid out its ambition and scope, emphasizing the need for standardization of experimental and computational methods [4]. The breast was notoriously absent from the initial list of biological systems presented by the founding consortium (as were other reproductive tissues), but this was to change a couple of months later, when Kai Kessenbrock (University of California, Irvine), Devon Lawson (University of California, Irvine), and Nicholas Navin (University of Texas MD Anderson Cancer Center) met at an HCA General Meeting, held at Stanford University in February 2017. Here, CZI introduced the project, its organization as a scientist-led con-
影响因子：
64.8
作者：
Snyder, Michael P.;Gingeras, Thomas R.;Myers, Richard M.
通讯作者：
Myers, Richard M.
影响因子：
16.6
作者：
Bach K;Pensa S;Grzelak M;Hadfield J;Adams DJ;Marioni JC;Khaled WT
通讯作者：
Khaled WT
影响因子：
3.8
作者：
Degnim, Amy C.;Visscher, Daniel W.;Hartmann, Lynn C.
通讯作者：
Hartmann, Lynn C.