The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool.
The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool.
复制标题
DOI：
10.1371/journal.pone.0010034
复制
发表时间：
2010-04-07
期刊：
影响因子：
3.7
通讯作者：
Brisse S
中科院分区：
文献类型：
--
作者：
Diancourt L;Passet V;Nemec A;Dijkshoorn L;Brisse S
鲍曼不动杆菌多重耐药菌株引起的医院感染暴发在世界范围内引起越来越多的关注。虽然已有报道称特定的暴发菌株在地理上分布广泛，但对鲍曼不动杆菌克隆群的多样性和系统发育相关性知之甚少。对154株鲍曼不动杆菌的7个看家基因(总计2976个核苷酸)的内部部分进行了测序，这些菌株涵盖了广泛的已知多样性，包括先前公认的国际克隆的代表，以及19个其他不动杆菌物种的代表。有限的多样性和星状的系统发育表明，鲍曼假丝酵母是一个基因紧凑的物种，在最近遭受了严重的瓶颈，可能与有限的生态位有关。鲍曼不动杆菌与其近亲(基因组种13TU)和其他不动杆菌种有很好的区分。多位点序列分型分析表明，先前公认的国际克隆I到III对应于三个克隆复合体，每个克隆复合体由一个中心的优势基因和少数单基因座变异组成，这是最近克隆扩张的标志。虽然在这些菌株和一个新的国际克隆(ST15)中几乎普遍存在抗菌素耐药性，但其他基因型的菌株大多敏感。这种二分法表明，抗菌素耐药性是一个主要的选择优势，推动了这些医院感染的高问题病原体的持续快速克隆性扩张。
Outbreaks of hospital infections caused by multidrug resistant Acinetobacter baumannii strains are of increasing concern worldwide. Although it has been reported that particular outbreak strains are geographically widespread, little is known about the diversity and phylogenetic relatedness of A. baumannii clonal groups. Sequencing of internal portions of seven housekeeping genes (total 2,976 nt) was performed in 154 A. baumannii strains covering the breadth of known diversity and including representatives of previously recognized international clones, and in 19 representatives of other Acinetobacter species. Restricted amounts of diversity and a star-like phylogeny reveal that A. baumannii is a genetically compact species that suffered a severe bottleneck in the recent past, possibly linked to a restricted ecological niche. A. baumannii is neatly demarcated from its closest relative (genomic species 13TU) and other Acinetobacter species. Multilocus sequence typing analysis demonstrated that the previously recognized international clones I to III correspond to three clonal complexes, each made of a central, predominant genotype and few single locus variants, a hallmark of recent clonal expansion. Whereas antimicrobial resistance was almost universal among isolates of these and a novel international clone (ST15), isolates of the other genotypes were mostly susceptible. This dichotomy indicates that antimicrobial resistance is a major selective advantage that drives the ongoing rapid clonal expansion of these highly problematic agents of nosocomial infections.
登录
查看更多内容
影响因子：
3.3
作者：
Didelot, Xavier;Falush, Daniel
通讯作者：
Falush, Daniel
影响因子：
9.4
作者：
DIJKSHOORN, L;AUCKEN, HM;PITT, TL
通讯作者：
PITT, TL
DOI：
10.1016/0769-2609(87)90042-1
发表时间：
1987-09-01
期刊：
ANNALES DE L INSTITUT PASTEUR-MICROBIOLOGIE
影响因子：
--
作者：
BOUVET, PJM;GRIMONT, PAD
通讯作者：
GRIMONT, PAD
影响因子：
2.8
作者：
Gundi, Vijay A. K. B.;Dijkshoorn, Lenie;La Scola, Bernard
通讯作者：
La Scola, Bernard
影响因子：
9.4
作者：
Bartual, SG;Seifert, H;Rodríguez-Valera, F
通讯作者：
Rodríguez-Valera, F