Multiplex Serology for Measurement of IgG Antibodies Against Eleven Infectious Diseases in a National Serosurvey: Haiti 2014-2015.
Multiplex Serology for Measurement of IgG Antibodies Against Eleven Infectious Diseases in a National Serosurvey: Haiti 2014-2015.
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DOI：
10.3389/fpubh.2022.897013
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发表时间：
2022
影响因子：
5.2
通讯作者：
Rogier, Eric William
中科院分区：
文献类型：
--
作者：
Chan, YuYen;Martin, Diana;Mace, Kimberly E.;Jean, Samuel E.;Stresman, Gillian;Drakeley, Chris;Chang, Michelle A.;Lemoine, Jean F.;Udhayakumar, Venkatachalam;Lammie, Patrick J.;Priest, Jeffrey W.;Rogier, Eric William
多种疾病的综合监测可以有效利用资源，有利于国家公共卫生计划。IgG抗体的检测通常表明先前暴露于病原体，但也可能用于评估活动性感染状态。最近开发了血清学多重微珠测定，以同时评价对多种抗原靶标的暴露。海地是加勒比地区的一个岛国，有多种地方性传染病，其中许多疾病的人口流行率或感染率数据很少。2014年12月至2015年2月在海地进行了全国范围的血清调查。滤纸血样（n = 4，438）从117个地点的参与者中收集，并在含有来自11种病原体的15种不同抗原的多重珠粒测定法上测定IgG抗体：恶性疟原虫、刚地弓形虫、淋巴丝虫病蛔虫、粪类圆线虫、基孔肯雅病毒、登革热病毒、沙眼衣原体、梅毒螺旋体、肠致病性大肠杆菌、溶组织内阿米巴、和隐孢子虫。不同比例的海地研究人群对不同的目标呈IgG血清阳性，抗原来自T。gondii、弓形虫C. parvum、登革病毒、基孔肯雅病毒和C.沙眼的血清阳性率最高。对T.梅毒和淋巴丝虫病的感染率最低，所有样本中对来自这些病原体的抗原的IgG血清反应阳性的不到5%。除基孔肯雅病毒和大肠杆菌抗原外，所有抗原的血清阳性率和IgG水平均随着年龄的增长而明显增加。溶组织剂参数模型能够估计海地居民每年的血清转化率和IgG获得率。多重血清学检测可以提供关于人群暴露于不同传染病的丰富信息。目前的海地研究包括虫媒病毒、寄生虫和细菌感染性疾病的IgG靶标，代表多种不同的宿主传播模式。在海地，对其中一些传染病的血清学研究很少或完全没有发表。海地的疾病负担与年龄和地点有关的明确趋势可供国家方案和合作伙伴用于后续研究、资源分配和干预规划。
Integrated surveillance for multiple diseases can be an efficient use of resources and advantageous for national public health programs. Detection of IgG antibodies typically indicates previous exposure to a pathogen but can potentially also serve to assess active infection status. Serological multiplex bead assays have recently been developed to simultaneously evaluate exposure to multiple antigenic targets. Haiti is an island nation in the Caribbean region with multiple endemic infectious diseases, many of which have a paucity of data for population-level prevalence or exposure. A nationwide serosurvey occurred in Haiti from December 2014 to February 2015. Filter paper blood samples (n = 4,438) were collected from participants in 117 locations and assayed for IgG antibodies on a multiplex bead assay containing 15 different antigens from 11 pathogens: Plasmodium falciparum, Toxoplasma gondii, lymphatic filariasis roundworms, Strongyloides stercoralis, chikungunya virus, dengue virus, Chlamydia trachomatis, Treponema pallidum, enterotoxigenic Escherichia coli, Entamoeba histolytica, and Cryptosporidium parvum. Different proportions of the Haiti study population were IgG seropositive to the different targets, with antigens from T. gondii, C. parvum, dengue virus, chikungunya virus, and C. trachomatis showing the highest rates of seroprevalence. Antibody responses to T. pallidum and lymphatic filariasis were the lowest, with <5% of all samples IgG seropositive to antigens from these pathogens. Clear trends of increasing seropositivity and IgG levels with age were seen for all antigens except those from chikungunya virus and E. histolytica. Parametric models were able to estimate the rate of seroconversion and IgG acquisition per year for residents of Haiti. Multiplex serological assays can provide a wealth of information about population exposure to different infectious diseases. This current Haitian study included IgG targets for arboviral, parasitic, and bacterial infectious diseases representing multiple different modes of host transmission. Some of these infectious diseases had a paucity or complete absence of published serological studies in Haiti. Clear trends of disease burden with respect to age and location in Haiti can be used by national programs and partners for follow-up studies, resource allocation, and intervention planning.
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影响因子：
3.8
作者：
Fujii Y;Kaneko S;Nzou SM;Mwau M;Njenga SM;Tanigawa C;Kimotho J;Mwangi AW;Kiche I;Matsumoto S;Niki M;Osada-Oka M;Ichinose Y;Inoue M;Itoh M;Tachibana H;Ishii K;Tsuboi T;Yoshida LM;Mondal D;Haque R;Hamano S;Changoma M;Hoshi T;Kamo K;Karama M;Miura M;Hirayama K
通讯作者：
Hirayama K
影响因子：
14.2
作者：
Hill, D;Dubey, JP
通讯作者：
Dubey, JP
DOI：
10.1179/2047773211y.0000000008
发表时间：
2011-12-01
影响因子：
--
作者：
Joseph, H.;Maiava, F.;Melrose, W.
通讯作者：
Melrose, W.
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
影响因子：
3.8
作者：
Kim, Jessica S.;Oldenburg, Catherine E.;Martin, Diana L.
通讯作者：
Martin, Diana L.