Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models.
Serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models.
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DOI：
10.1371/journal.pntd.0003040
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发表时间：
2014
影响因子：
3.8
通讯作者：
Hirayama K
中科院分区：
文献类型：
--
作者：
Fujii Y;Kaneko S;Nzou SM;Mwau M;Njenga SM;Tanigawa C;Kimotho J;Mwangi AW;Kiche I;Matsumoto S;Niki M;Osada-Oka M;Ichinose Y;Inoue M;Itoh M;Tachibana H;Ishii K;Tsuboi T;Yoshida LM;Mondal D;Haque R;Hamano S;Changoma M;Hoshi T;Kamo K;Karama M;Miura M;Hirayama K
防治传染病，包括被忽视的热带病的战略将取决于发展可靠的流行病监测方法。为了建立一个简单实用的血清阳性率检测系统，我们开发了一种基于微球的多重免疫测定系统，并使用在肯尼亚获得的样本进行了效用评估。我们开发了一种基于微球的免疫测定系统，以同时测量针对来自6种病原体的8种抗原的血浆抗体（IgG）水平：溶组织内阿米巴（C-IgL），杜氏利什曼原虫（KRP 42），弓形虫（SAG 1），班氏吴策线虫（SXP 1），HIV（gag，gp120和gp41），霍乱弧菌（霍乱毒素）。使用适当的对照样品对试验系统进行验证。该检测系统应用于从肯尼亚沿海和西部地区的两个健康和人口监测系统（HDSS）队列中随机选择的一般人群中收集的3411份血液样本。通过有限混合物模型在数学上定义每种抗原的免疫测定值分布，并优化截止值。每种抗原的灵敏度和特异性范围为71 - 100%。来自夸莱和Mbita HDSS站点的每种病原体的血清阳性率分别如下：HIV，3.0%和20.1%; donovani，12.6%和17.3%;溶组织T.弓形虫感染率分别为30.9%和28.2%。W. bancrofti和霍乱弧菌的感染率相对较高，特别是在儿童中。结果可能受W. bancrofti-SXP1和其它寄生虫感染;以及霍乱毒素和肠致病性E.大肠杆菌（ETEC）。基于微球的多血清学检测系统可以为全面掌握NTD的流行病学特征提供机会。通过添加感兴趣的病原体和抗原，可以建立优化的定制高质量程序，以利用有限的资源有效控制非洲的NTD。监测被忽视的热带病的分布是控制其在非洲蔓延的关键。目前，这种监测是对每一个NTD独立进行的。为了解决这个问题，我们开发了一个基于微球的系统，允许同时测量IgG抗体水平的抗原从六种传染病：溶组织内阿米巴，杜氏利什曼原虫，弓形虫，班氏吴策线虫，艾滋病病毒，霍乱弧菌。使用这个系统，我们进行了血清学调查，使用两个健康和人口监测系统（HDSS）在沿海和肯尼亚西部地区。我们根据性别和年龄组随机选择了4，600人，其中3，411人同意参加研究。参与者对每个抗原的反应性和血清阳性和阴性对照的反应性的分布的数学分析表明，该系统可用于监测感染，特别是与HIV，丝虫病，弓形虫病，利什曼病和阿米巴病相关的感染。为了在非洲实际开发和最终实施实际项目，可以添加感兴趣的病原体和抗原，以优化定制监测项目。
A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in Kenya. We developed a microsphere-based immuno-assay system to simultaneously measure the individual levels of plasma antibody (IgG) against 8 antigens derived from 6 pathogens: Entamoeba histolytica (C-IgL), Leishmania donovani (KRP42), Toxoplasma gondii (SAG1), Wuchereria bancrofti (SXP1), HIV (gag, gp120 and gp41), and Vibrio cholerae (cholera toxin). The assay system was validated using appropriate control samples. The assay system was applied for 3411 blood samples collected from the general population randomly selected from two health and demographic surveillance system (HDSS) cohorts in the coastal and western regions of Kenya. The immunoassay values distribution for each antigen was mathematically defined by a finite mixture model, and cut-off values were optimized. Sensitivities and specificities for each antigen ranged between 71 and 100%. Seroprevalences for each pathogen from the Kwale and Mbita HDSS sites (respectively) were as follows: HIV, 3.0% and 20.1%; L. donovani, 12.6% and 17.3%; E. histolytica, 12.8% and 16.6%; and T. gondii, 30.9% and 28.2%. Seroprevalences of W. bancrofti and V. cholerae showed relatively high figures, especially among children. The results might be affected by immunological cross reactions between W. bancrofti-SXP1 and other parasitic infections; and cholera toxin and the enterotoxigenic E. coli (ETEC), respectively. A microsphere-based multi-serological assay system can provide an opportunity to comprehensively grasp epidemiological features for NTDs. By adding pathogens and antigens of interest, optimized made-to-order high-quality programs can be established to utilize limited resources to effectively control NTDs in Africa. Monitoring the distribution of neglected tropical diseases (NTDs) is a key to controlling their spread in Africa. Currently, such surveillance is conducted independently for each NTD. To tackle this problem, we developed a microsphere-based system to permit simultaneous measurement of IgG antibody levels for antigens from six infectious diseases: Entamoeba histolytica, Leishmania donovani, Toxoplasma gondii, Wuchereria bancrofti, HIV, and Vibrio cholerae. Using this system, we conducted a serological survey using two health and demographic surveillance system (HDSS) areas in coastal and western Kenya. We randomly selected 4,600 individuals according to sex and age group, of whom 3411 agreed to participate in the study. Mathematical analyses of the distributions of the participants' reactivity to each antigen and the reactivity of the sero-positive and -negative controls indicated that this system could be used to monitor infections, especially, those associated with HIV, filariasis, toxoplasmosis, leishmaniasis, and amebiasis. For the practical development and eventual implementation of actual programs in Africa, pathogens and antigens of interest can be added to optimize made-to-order monitoring programs.
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DOI：
10.1016/s0035-9203(03)80012-3
发表时间：
2003-09-01
影响因子：
2.2
作者：
Marlet, MVL;Ritmeijer, K;Davidson, RN
通讯作者：
Davidson, RN
影响因子：
--
作者：
Pickering, Jerry W.;Larson, Matthew T.;Hill, Harry R.
通讯作者：
Hill, Harry R.
影响因子：
3.2
作者：
Njenga SM;Mwandawiro CS;Wamae CN;Mukoko DA;Omar AA;Shimada M;Bockarie MJ;Molyneux DH
通讯作者：
Molyneux DH
影响因子：
2.3
作者：
Everitt, B S
通讯作者：
Everitt, B S
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.