tasiRNA-ARF pathway moderates floral architecture in Arabidopsis plants subjected to drought stress.
tasiRNA-ARF pathway moderates floral architecture in Arabidopsis plants subjected to drought stress.
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DOI：
10.1155/2014/303451
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发表时间：
2014
影响因子：
--
通讯作者：
Seki M
中科院分区：
文献类型：
--
作者：
Matsui A;Mizunashi K;Tanaka M;Kaminuma E;Nguyen AH;Nakajima M;Kim JM;Nguyen DV;Toyoda T;Seki M
在植物中，miRNAs和siRNAs，如transacting siRNAs（ta-siRNAs），通过不同的调节机制影响它们的靶标。本研究利用454 DNA测序技术分析了干旱、低温和高盐胁迫下拟南芥小分子RNA（smRNAs）的表达谱。在干旱和高盐胁迫下，三组ta-siRNAs（TAS1、TAS2和TAS3）及其前体的表达在拟南芥中下调。ta-siRNA合成突变体和逃避tasiRNA-ARF靶标的突变的ARF 3过表达植物的分析表明，在干旱和高盐胁迫下，植物中的短雄蕊阻碍了自花授粉。干旱胁迫和非胁迫条件下的RDR6和野生型植物的花蕾的微阵列分析表明，花发育和生长素反应相关基因的表达受到干旱胁迫和RDR6突变的影响。本研究的总体结果表明，tasiRNA-ARF通过微调花发育相关基因和生长素反应相关基因的表达变化，参与维持植物在胁迫条件下花的正常形态建成。
In plants, miRNAs and siRNAs, such as transacting siRNAs (ta-siRNAs), affect their targets through distinct regulatory mechanisms. In this study, the expression profiles of small RNAs (smRNAs) in Arabidopsis plants subjected to drought, cold, and high-salinity stress were analyzed using 454 DNA sequencing technology. Expression of three groups of ta-siRNAs (TAS1, TAS2, and TAS3) and their precursors was downregulated in Arabidopsis plants subjected to drought and high-salinity stress. Analysis of ta-siRNA synthesis mutants and mutated ARF3-overexpressing plants that escape the tasiRNA-ARF target indicated that self-pollination was hampered by short stamens in plants under drought and high-salinity stress. Microarray analysis of flower buds of rdr6 and wild-type plants under drought stress and nonstressed conditions revealed that expression of floral development- and auxin response-related genes was affected by drought stress and by the RDR6 mutation. The overall results of the present study indicated that tasiRNA-ARF is involved in maintaining the normal morphogenesis of flowers in plants under stress conditions through fine-tuning expression changes of floral development-related and auxin response-related genes.
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影响因子：
3.8
作者：
Cheptou, PO;Berger, A;Escarre, J
通讯作者：
Escarre, J
影响因子：
7.4
作者：
Lee, Sun-ji;Kang, Jung-youn;Kim, Soo Young
通讯作者：
Kim, Soo Young
影响因子：
7.4
作者：
Miller, Gad;Suzuki, Nobuhiro;Mittler, Ron
通讯作者：
Mittler, Ron
miRNA 和 siRNA 在植物生物和非生物胁迫反应中的作用。
DOI：
10.1016/j.bbagrm.2011.05.001
发表时间：
2012-02
影响因子：
4.7
作者：
Khraiwesh, Basel;Zhu, Jian-Kang;Zhu, Jianhua
通讯作者：
Zhu, Jianhua
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y