Multidrug Resistance and Virulence Factors of Escherichia coli Harboring Plasmid-Mediated Colistin Resistance: mcr-1 and mcr-3 Genes in Contracted Pig Farms in Thailand.
Multidrug Resistance and Virulence Factors of Escherichia coli Harboring Plasmid-Mediated Colistin Resistance: mcr-1 and mcr-3 Genes in Contracted Pig Farms in Thailand.
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DOI：
10.3389/fvets.2020.582899
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发表时间：
2020
影响因子：
3.2
通讯作者：
Prapasarakul N
中科院分区：
文献类型：
--
作者：
Khine NO;Lugsomya K;Kaewgun B;Honhanrob L;Pairojrit P;Jermprasert S;Prapasarakul N
肠杆菌科中质粒介导的粘菌素耐药编码mcr基因家族的存在是全球关注的关键问题之一。在家畜饲养中使用粘菌素被认为是mcr基因传播的原因，并对公众健康产生影响。本研究的目的是检测频率和毒力基因的mcr阳性大肠杆菌（MCRPE）的粪便样本中健康的猪在泰国的合同养殖系统。共有696个混合样本来自泰国6个地区49个省的80个农场。耐多粘菌素E.采用MALDI-TOF质谱鉴定和微量肉汤稀释法进行药敏试验。使用自动药敏仪确定了基因图谱，并研究了mcr-1-5基因、系统发育组、复制子类型和毒力基因的遗传特征。在696份样品中，31份为阳性，其中E.含mcr-1或mcr-1和mcr-3的大肠杆菌感染率分别为4.45%和0.43%。系统发育组A和B1以及IncF和IncFIB复制子类型主要见于位于中心区域的MCRPE，对3-14种抗菌药物具有多重耐药特征。此外，31株分离株中有19株被鉴定为肠致病性E.大肠杆菌的STAP和STB（肠毒素编码基因）。因此，mcr阳性大肠杆菌的低携带率。大肠杆菌在健康猪的规模化养殖中检出。多药耐药MCRPE与其致病潜力之间的关联应引起关注。
The presence of the plasmid-mediated colistin resistance encoding mcr gene family in the Enterobacteriaceae is one of the crucial global concerns. The use of colistin in livestock rearing is believed to be the cause of mcr gene spreading and is of impact to public health. The objective of this research was to detect the frequency and virulent genes of mcr-positive Escherichia coli (MCRPE) in fecal samples from healthy pigs in a contract farming system across Thailand. A total of 696 pooled samples were derived from 80 farms, located in 49 provinces across six regions of Thailand. The colistin-resistant E. coli were identified by MALDI-TOF mass spectrometry and antimicrobial susceptibility testing by broth microdilution. The antibiogram was determined using an automated susceptibility machine, and the genetic characteristics were investigated for mcr-1–5 genes, phylogenetic group, replicon types, and virulent genes. In total, 31 of 696 samples were positive, with E. coli containing mcr-1 or combination of mcr-1 and mcr-3 with incidence of 4.45 and 0.43%. Phylogenetic groups A and B1 and the IncF and IncFIB replicon types were predominantly found in the MCRPE located in the central area, with multidrug-resistant traits against 3–14 types of antimicrobials. Additionally, 19 of 31 isolates identified as enterotoxigenic E. coli were with the stap and stb (enterotoxin-encoding genes). In conclusion, a low carriage rate of mcr-positive E. coli was detected in the large-scale farming of healthy pigs. The association between multidrug-resistant MCRPE and their pathogenic potential should be of concern.
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影响因子：
1.5
作者：
Casey, Thomas A.;Bosworth, Brad T.
通讯作者：
Bosworth, Brad T.
影响因子：
5.2
作者：
Duggett, Nicholas A.;Randall, Luke P.;Anjum, Muna F.
通讯作者：
Anjum, Muna F.
DOI：
10.1016/j.ijantimicag.2016.11.026
发表时间：
2017-03-01
影响因子：
10.8
作者：
Rhouma, Mohamed;Letellier, Ann
通讯作者：
Letellier, Ann
影响因子：
4.8
作者：
Forde BM;Zowawi HM;Harris PNA;Roberts L;Ibrahim E;Shaikh N;Deshmukh A;Sid Ahmed MA;Al Maslamani M;Cottrell K;Trembizki E;Sundac L;Yu HH;Li J;Schembri MA;Whiley DM;Paterson DL;Beatson SA
通讯作者：
Beatson SA
影响因子：
4.4
作者：
Leonard, Susan R.;Mammel, Mark K.;Lacher, David W.
通讯作者：
Lacher, David W.