Better method for detection of CD30: Immunohistochemistry or flow cytometry?
Better method for detection of CD30: Immunohistochemistry or flow cytometry?
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DOI：
10.3960/jslrt.21019
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发表时间：
2021-12-22
期刊：
影响因子：
--
通讯作者：
Tamaru JI
中科院分区：
文献类型：
--
作者：
Higashi M;Kikuchi J;Murakami C;Takayanagi N;Momose S;Kizaki M;Tamaru JI
我们比较了两种评估DLBCL和TCL标本中CD 30蛋白表达的方法，免疫组化（IHC）和流式细胞术（FCM），在123例DLBCL和28例TCL患者中使用相同的抗CD 30抗体（Ber-H2）克隆。FCM比IHC更敏感，特别是在低表达的病例中。在3例TCL和2例DLBCL中，两种方法的结果不一致。这些TCL病例中有2例为ALCL，1例为外周T细胞淋巴瘤（NOS），但无法排除ALCL。2例DLBCL中1例为DLBCL的间变性变异。数据表明，在几种情况下，虽然罕见，但通过FMC检测不到CD 30。因此，建议将免疫组化和流式细胞术相结合，用于可靠和定量检测CD 30。
We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expression. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.
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