Phylogenetic Classification and Functional Review of Autotransporters.
Phylogenetic Classification and Functional Review of Autotransporters.
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DOI：
10.3389/fimmu.2022.921272
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
--
作者：
自动转运蛋白是分子纳米机器的核心组件，可将货物蛋白输送穿过革兰氏阴性细菌的外膜。作为 V 型分泌系统的一部分，这一大蛋白家族通过促进表面粘附、生物膜形成、宿主定植和入侵以及细胞毒性和免疫调节，在控制细菌与其环境相互作用方面发挥着核心作用。因此，自转运蛋白是健康和发病机制的关键促进者，并能够与其他细菌合作或竞争。近年来，报道的自转运蛋白序列数量急剧增加，功能研究也稳步增加，这进一步将这些蛋白质与多种毒力表型联系起来。在这篇综述中，我们概述了我们目前对经典自转运蛋白（该蛋白超家族的原型）的了解。我们还对其功能域进行了系统发育分析，并为这一极其多样化的细菌蛋白组提出了一个新的分类系统。确定的十六个系统发育分区在明确表征的自转运蛋白之间建立了合理的关系，并为未表征的蛋白质的结构和功能预测提供信息，这可能指导未来的研究，旨在解决这组治疗上重要的细菌因子中多个未解答的方面。
Autotransporters are the core component of a molecular nano-machine that delivers cargo proteins across the outer membrane of Gram-negative bacteria. Part of the type V secretion system, this large family of proteins play a central role in controlling bacterial interactions with their environment by promoting adhesion to surfaces, biofilm formation, host colonization and invasion as well as cytotoxicity and immunomodulation. As such, autotransporters are key facilitators of fitness and pathogenesis and enable co-operation or competition with other bacteria. Recent years have witnessed a dramatic increase in the number of autotransporter sequences reported and a steady rise in functional studies, which further link these proteins to multiple virulence phenotypes. In this review we provide an overview of our current knowledge on classical autotransporter proteins, the archetype of this protein superfamily. We also carry out a phylogenetic analysis of their functional domains and present a new classification system for this exquisitely diverse group of bacterial proteins. The sixteen phylogenetic divisions identified establish sensible relationships between well characterized autotransporters and inform structural and functional predictions of uncharacterized proteins, which may guide future research aimed at addressing multiple unanswered aspects in this group of therapeutically important bacterial factors.
EHAJ的表征，EHAJ是一种新的自动转运蛋白，来自肠菌和肠病性大肠杆菌。
影响因子：
5.2
作者：
Easton DM;Totsika M;Allsopp LP;Phan MD;Idris A;Wurpel DJ;Sherlock O;Zhang B;Venturini C;Beatson SA;Mahony TJ;Cobbold RN;Schembri MA
通讯作者：
Schembri MA