Angiogenin stimulates ribosomal RNA transcription by epigenetic activation of the ribosomal DNA promoter.
Angiogenin stimulates ribosomal RNA transcription by epigenetic activation of the ribosomal DNA promoter.
复制标题
血管生成素通过核糖体 DNA 启动子的表观遗传激活刺激核糖体 RNA 转录
DOI：
10.1002/jcp.24477
复制
发表时间：
2014-04
影响因子：
5.6
通讯作者：
Hu, Guo-Fu
中科院分区：
文献类型：
--
作者：
Sheng, Jinghao;Yu, Wenhao;Gao, Xiangwei;Xu, Zhengping;Hu, Guo-Fu
血管生成素（ANG）经历核转位并促进核糖体RNA（rRNA）转录，从而促进细胞生长和增殖。然而，ANG刺激rRNA转录的作用方式尚不清楚。在这里，我们表明ANG增强了核糖体DNA（rDNA）启动子处RNA聚合酶I（Pol I）前起始复合物的形成。ANG结合在启动子的上游控制元件（UCE）处，并增强RNA Pol I以及选择性因子SL 1组分TAFI 48和TAFI 110的启动子占据。我们还表明，ANG通过启动子甲基化和组蛋白修饰的表观遗传激活增加了活跃转录rDNA的数量。ANG与组蛋白H3结合，抑制H3 K9甲基化，并激活rDNA启动子处的H3 K4甲基化以及H4乙酰化。这些数据表明，ANG刺激rRNA转录的机制之一是通过rDNA启动子的表观遗传激活。J.细胞。229：521-529，2014。© 2013 Wiley Periodicals，Inc.
Angiogenin (ANG) undergoes nuclear translocation and promotes ribosomal RNA (rRNA) transcription thereby enhancing cell growth and proliferation. However, the mode of action of ANG in stimulating rRNA transcription is unclear. Here, we show that ANG enhances the formation of RNA polymerase I (Pol I) pre‐initiation complex at the ribosomal DNA (rDNA) promoter. ANG binds at the upstream control element (UCE) of the promoter and enhances promoter occupancy of RNA Pol I as well as the selectivity factor SL1 components TAFI48 and TAFI110. We also show that ANG increases the number of actively transcribing rDNA by epigenetic activation through promoter methylation and histone modification. ANG binds to histone H3, inhibits H3K9 methylation, and activates H3K4 methylation as well as H4 acetylation at the rDNA promoter. These data suggest that one of the mechanisms by which ANG stimulates rRNA transcription is through an epigenetic activation of rDNA promoter. J. Cell. Physiol. 229: 521–529, 2014. © 2013 Wiley Periodicals, Inc.
登录
查看更多内容
DOI：
10.1158/1078-0432.ccr-08-2593
发表时间：
2009-03-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Ibaragi S;Yoshioka N;Li S;Hu MG;Hirukawa S;Sadow PM;Hu GF
通讯作者：
Hu GF
DOI：
10.1073/pnas.94.6.2204
发表时间：
1997-03-18
影响因子：
11.1
作者：
Hu, GF;Riordan, JF;Vallee, BL
通讯作者：
Vallee, BL
影响因子：
2.9
作者：
FETT, JW;STRYDOM, DJ;VALLEE, BL
通讯作者：
VALLEE, BL
DOI：
10.1006/bbrc.1994.2391
发表时间：
1994-09-30
影响因子：
3.1
作者：
MOROIANU, J;RIORDAN, JF
通讯作者：
RIORDAN, JF
DOI：
10.1158/1541-7786.mcr-08-0137
发表时间：
2009-03
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Ibaragi S;Yoshioka N;Kishikawa H;Hu JK;Sadow PM;Li M;Hu GF
通讯作者：
Hu GF