Feasibility of an enhanced washing process to extract PBDEs/heavy metals/antibiotics from antibiotic resistance gene-affected soil with aqueous DNA followed by microbial augmentation
Feasibility of an enhanced washing process to extract PBDEs/heavy metals/antibiotics from antibiotic resistance gene-affected soil with aqueous DNA followed by microbial augmentation
复制标题
强化洗涤工艺的可行性，用水性 DNA 从受抗生素抗性基因影响的土壤中提取 PBDEs/重金属/抗生素，然后进行微生物强化
DOI：
10.1007/s11368-015-1291-3
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发表时间：
2016-03
影响因子：
3.6
通讯作者：
Xin Jiang
中科院分区：
文献类型：
--
作者：
Ye Mao;Sun Mingming;Wan Jinzhong;Zhao Yu;Xie Shanni;Tian Da;Hu Feng;Li Huixin;Zong Lianggang;Kengara Fredrick Orori;Xin Jiang
研究目的采用改良的土壤淋洗方法，研究DNA溶液提取高丰度抗生素抗性基因（antibiotics resistance gene，ARGs）污染土壤中多溴联苯醚（polybrominated diphenyl ethers，PBDEs）、铜（Cu）、铅（Pb）、四环素（tetracycline）和磺胺嘧啶（sulfadiazine）的可能性，结果和讨论用15 g L− 1 DNA溶液加超声处理连续两次洗涤，结果表明，在25 kHz，15 min条件下，BDE-28、BDE-47、Cu、Pb、四环素和磺胺嘧啶的提取率分别为80.6%、85.2%、64.7%、60.3%、100%。同时，ARG丰度下降到10−7-10−8（ARG拷贝/16 S拷贝）的水平。此外，对第二次洗涤的土壤接种多溴联苯醚降解菌并施用营养物3个月的联合处理导致残留的BDE 28和BDE 47进一步降解。高通量测序技术分析和重金属稳定性模型评价表明，该组合净化技术修复混合污染土壤是一种环境友好、有效的技术。
PurposeRemediation of polybrominated diphenyl ethers (PBDEs)/heavy metals/antibiotics mixed contaminated soil with high abundance of antibiotic resistance genes (ARGs) has being greatly challenging.Materials and methodsAn elevated soil washing procedure was developed to investigate the potential of applying DNA solution to extract PBDEs, Cu, Pb, tetracycline, and sulfadiazine from a high abundance of ARG-affected soil, followed by microbial augmentation to restore the microbiological functioning of washed soil.Results and discussionTwo successive washings with 15 g L−1DNA solution plus ultrasonication (25 kHz for 15 min) were optimal for extracting 80.6 % BDE28, 85.2 % BDE47, 64.7 % Cu, 60.3 % Pb, 100 % tetracycline, and 100 % sulfadiazine. Simultaneously, the ARG abundance decreased to the level of 10−7–10−8(ARGs copies/16S copies). Moreover, combined treatment with PBDE-degrading bacteria inoculation and nutrient application for 3 months to the second washed soil resulted in the further degradation of residual BDE28 and BDE47. Furthermore, the bacterial community composition was restored and the transfer risk of residual pollutants was limited, as indicated by high-throughput sequencing technology analysis and heavy metals stability model evaluation.ConclusionsThe combined cleanup technology is an environmentally friendly and effective technology to remediate mixed contaminated soils.
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影响因子：
3.1
作者：
Fei Yang;Yiming Long;Rui Shen;Chunyan Chen;Di Pan;Qianli Zhang;Q. Cai;S. Yao
通讯作者：
Fei Yang;Yiming Long;Rui Shen;Chunyan Chen;Di Pan;Qianli Zhang;Q. Cai;S. Yao
影响因子：
11.8
作者：
Yanguo Teng;Jin Wu;Sijin Lu;Yeyao Wang;X. Jiao;Liuting Song
通讯作者：
Yanguo Teng;Jin Wu;Sijin Lu;Yeyao Wang;X. Jiao;Liuting Song
影响因子：
8.8
作者：
Jho, Eun Hea;Im, Jinwoo;Nam, Kyoungphile
通讯作者：
Nam, Kyoungphile
影响因子：
13.6
作者：
G. Ji;Guohui Zhou
通讯作者：
G. Ji;Guohui Zhou
DOI：
10.1073/pnas.1222743110
发表时间：
2013-02-26
影响因子：
11.1
作者：
Zhu, Yong-Guan;Johnson, Timothy A.;Tiedje, James M.
通讯作者：
Tiedje, James M.