AUXIN RESPONSE FACTOR 3 integrates the functions of AGAMOUS and APETALA2 in floral meristem determinacy.

AUXIN RESPONSE FACTOR 3 integrates the functions of AGAMOUS and APETALA2 in floral meristem determinacy.
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DOI:
10.1111/tpj.12658
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发表时间:
2014-11
期刊:
The Plant journal : for cell and molecular biology
影响因子:
--
通讯作者:
Chen X
Chen X
中科院分区:
其他
文献类型:
--
作者:
Liu X;Dinh TT;Li D;Shi B;Li Y;Cao X;Guo L;Pan Y;Jiao Y;Chen X

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在拟南芥中,生长素应答因子3(ARF 3)属于生长素应答因子(ARF)家族,其调节生长素应答基因的表达。已知ARF 3在叶极性特化和雌蕊群图案形成中起作用。在这项研究中,我们发现了一个以前未知的作用,ARF 3在花分生组织(FM)的确定性,通过分离和表征的突变体的ARF 3,增强FM确定性缺陷的agamous(ag)-10,弱ag等位基因。FM确定性的主要参与者包括WUSCHEL(WUS),一个对FM维持至关重要的基因,以及AG和APETALA 2(AP 2),它们分别通过抑制和促进WUS表达来调节FM确定性。我们发现,ARF 3通过抑制WUS表达赋予FM确定性,并与WUS位点部分以AG依赖的方式相关。我们证明了ARF 3是AP 2的直接靶点,并部分介导了AP 2在FM确定性中的功能。ARF 3在花器官原基中表现出动态和复杂的表达模式;改变模式空间上损害FM确定性。这项研究揭示了ARF 3在FM确定性中的作用,并揭示了控制FM确定性的遗传网络中基因之间的关系。
In Arabidopsis, AUXIN RESPONSE FACTOR 3 (ARF3) belongs to the auxin response factor (ARF) family that regulates the expression of auxin-responsive genes. ARF3 is known to function in leaf polarity specification and gynoecium patterning. In this study, we discovered a previously unknown role of ARF3 in floral meristem (FM) determinacy through the isolation and characterization of a mutant of ARF3 that enhanced the FM determinacy defects of agamous (ag)-10, a weak ag allele. Central players in FM determinacy include WUSCHEL (WUS), a gene critical for FM maintenance, and AG and APETALA2 (AP2), which regulate FM determinacy by repression and promotion of WUS expression, respectively. We showed that ARF3 confers FM determinacy through repression of WUS expression, and associates with the WUS locus in part in an AG-dependent manner. We demonstrated that ARF3 is a direct target of AP2 and partially mediates AP2’s function in FM determinacy. ARF3 exhibits dynamic and complex expression patterns in floral organ primordia; altering the patterns spatially compromised FM determinacy. This study uncovered a role for ARF3 in FM determinacy and revealed relationships among genes in the genetic network governing FM determinacy.
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