Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry.
Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry.
复制标题
通过二维电泳和 MALDI-TOF 质谱法鉴定人食管永生化细胞系和癌细胞系之间的差异表达蛋白。
DOI：
10.3748/wjg.v8.i5.777
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发表时间：
2002-10
期刊：
影响因子：
--
通讯作者：
Li EM
中科院分区：
文献类型：
--
作者：
Xiong XD;Xu LY;Shen ZY;Cai WJ;Luo JM;Han YL;Li EM
AIM
To identify the differentially expressed proteins between the human immortalized esophageal epithelial cell line (SHEE) and the malignant transformed esophageal carcinoma cell line (SHEEC), and to explore new ways for studying esophageal carcinoma associated genes.
METHODS
SHEE and SHEEC cell lines were used to separate differentially expressed proteins by two-dimensional electrophoresis. The silver-stained 2-D gels was scanned with EDAS290 digital camera system and analyzed with the PDQuest 6.2 Software. Six spots in which the differentially expressed protein was more obvious were selected and analyzed with matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).
RESULTS
There were 107+/-4.58 and 115+/-9.91 protein spots observed in SHEE and SHEEC respectively, and the majority of these spots between the two cell lines matched each other (r=0.772), only a few were expressed differentially. After analyzed by MALDI-TOF-MS and database search for the six differentially expressed proteins, One new protein as well as other five sequence-known proteins including RNPEP-like protein, human rRNA gene upstream sequence binding transcription factor, uracil DNA glycosylase, Annexin A2 and p300/CBP-associated factor were preliminarily identified.
CONCLUSION
These differentially expressed proteins might play an importance role during malignant transformation of SHEEC from SHEE. The identification of these proteins may serve as a new way for studying esophageal carcinoma associated genes.
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影响因子：
11.2
作者：
A. Vercoutter‐Edouart;J. Lemoine;X. Bourhis;H. Louis;B. Boilly;V. Nurcombe;F. Révillion;J. Peyrat-J.-Peyr
通讯作者：
A. Vercoutter‐Edouart;J. Lemoine;X. Bourhis;H. Louis;B. Boilly;V. Nurcombe;F. Révillion;J. Peyrat-J.-Peyr
人乳头瘤病毒诱导食管上皮细胞多期癌变中端粒酶活性与恶性表型的比较研究
DOI：
10.3892/ijmm.8.6.633
发表时间：
2001-12
期刊：
Int J Mol Med,
影响因子：
--
作者：
Shen ZY;Xu LY;Li C;Cai WJ;Shen J;Chen JY;Zeng Y
通讯作者：
Zeng Y
影响因子：
4.3
作者：
Zhongping Gu;Yun-jie Wang;Jin-ge Li;Yong-an Zhou
通讯作者：
Zhongping Gu;Yun-jie Wang;Jin-ge Li;Yong-an Zhou
影响因子：
2.9
作者：
R. Rakwal;G. Agrawal;M. Yonekura
通讯作者：
R. Rakwal;G. Agrawal;M. Yonekura
影响因子：
2.9
作者：
J. Beckers;P. Boček
通讯作者：
J. Beckers;P. Boček