Photodynamic therapy using 5-aminolaevulinic acid for experimental pancreatic cancer--prolonged animal survival.
Photodynamic therapy using 5-aminolaevulinic acid for experimental pancreatic cancer--prolonged animal survival.
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DOI：
10.1038/bjc.1994.288
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发表时间：
1994-08
影响因子：
8.8
通讯作者：
Bown, S. G.
中科院分区：
文献类型：
--
作者：
Regula, J.;Ravi, B.;Bedwell, J.;Macrobert, A. J.;Bown, S. G.
Experimental studies have been carried out using 5-aminolaevulinic acid (ALA) to induce transient porphyrin photosensitisation for photodynamic therapy (PDT) in a pancreatic cancer model in Syrian golden hamsters. ALA was given either intravenously or orally (in bolus or fractionated doses) with the laser light delivered by means of a bare fibre touching the tissue surface or external irradiation using a light-integrating cylindrical applicator. Animals were killed 1-24 h after ALA administration for pharmacokinetic studies and 3-7 days after light exposure to study PDT-induced necrosis. A separate survival study was also performed after a fractionated oral dose of ALA and external irradiation. Protoporphyrin IX sensitisation in the tumour tissue as measured by quantitative fluorescence microscopy was highest after intravenous administration of 200 mg kg-1 ALA and then in decreasing order after oral fractionated and oral bolus doses (both 400 mg kg-1). Laser light application at 630 nm to give 12-50 J from the bare fibre or 50 J cm-2 using surface illumination with the cylindrical applicator resulted in tumour necrosis up to 8 mm in depth. In larger tumours a rim of viable tumour was observed on the side opposite to illumination. In a randomised study, survival of treated animals was significantly longer than in the untreated control group (log-rank test, P < 0.02), although all animals died of recurrent tumour. This technique shows promise in the treatment of small volumes of tumour in the pancreas.
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DOI：
10.1111/j.1365-2362.1992.tb01482.x
发表时间：
1992-06-01
影响因子：
5.5
作者：
MUSTAJOKI, P;TIMONEN, K;TENHUNEN, R
通讯作者：
TENHUNEN, R
影响因子：
8.8
作者：
NUUTINEN, PJO;CHATLANI, PT;BOWN, SG
通讯作者：
BOWN, SG
影响因子：
2.4
作者：
DOUGHERTY, TJ;COOPER, MT;MANG, TS
通讯作者：
MANG, TS
影响因子：
29.4
作者：
VANHILLEGERSBERG, R;VANDENBERG, JWO;WILSON, JHP
通讯作者：
WILSON, JHP
影响因子：
8.8
作者：
Barr H;Tralau CJ;MacRobert AJ;Krasner N;Boulos PB;Clark CG;Bown SG
通讯作者：
Bown SG