TOF-SIMS 3D imaging of native and non-native species within HeLa cells.
TOF-SIMS 3D imaging of native and non-native species within HeLa cells.
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DOI：
10.1021/ac402288d
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发表时间：
2013-11-19
影响因子：
7.4
通讯作者：
Castner, David G.
中科院分区：
文献类型：
--
作者：
Brison, Jeremy;Robinson, Michael A.;Benoit, Danielle S. W.;Muramoto, Shin;Stayton, Patrick S.;Castner, David G.
In this study a non-native chemical species, bromodeoxyuridine (BrdU), was imaged within single HeLa cells using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Z-corrected 3D images were reconstructed that accurately portray the distribution of intracellular BrdU as well as other intracellular structures. The BrdU was localized to the nucleus of cells, whereas structures composed of CxHyOz− were located in bundles on the periphery of cells. The CxHyOz− sub-cellular features had a spatial resolution at or slightly below a micron (900nm), as defined by the distance between the 16 and 84% intensities in a line scan across the edge of the features. Additionally, important parameters influencing the quality of the HeLa cell 3D images were investigated. Atomic force microscopy measurements revealed that the HeLa cells were sputtered at a rate of approximately 4 nm per 1013 C60+ ions/cm2 at 10 keV and a 45° incident angle. Optimal 3D images were acquired using a Bi3+ liquid metal ion gun operating in the simultaneous high mass and spatial resolution mode.
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影响因子：
3.7
作者：
Bouneau, S;Brunelle, A;Andersen, HH
通讯作者：
Andersen, HH
影响因子：
16.6
作者：
Breitenstein, Daniel;Rommel, Christina E.;Hagenhoff, Birgit
通讯作者：
Hagenhoff, Birgit
影响因子：
2
作者：
Fletcher, John S.;Rabbani, Sadia;Vickerman, John C.
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Vickerman, John C.
影响因子：
4.3
作者：
Gutleb, Arno C.;Freitas, Jaime;Audinot, Jean-Nicolas
通讯作者：
Audinot, Jean-Nicolas
影响因子：
6.7
作者：
Breitenstein, D.;Rommel, C. E.;Hagenhoff, B.
通讯作者：
Hagenhoff, B.