Nitric oxide responses in Arabidopsis hypocotyls are mediated by diverse phytohormone pathways.
Nitric oxide responses in Arabidopsis hypocotyls are mediated by diverse phytohormone pathways.
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DOI：
10.1093/jxb/ery286
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发表时间：
2018-10-12
影响因子：
6.9
通讯作者：
León J
中科院分区：
文献类型：
--
作者：
Castillo MC;Coego A;Costa-Broseta Á;León J
控制黄化幼苗下胚轴生长的NO敏感机制需要乙烯、独脚金内酯、水杨酸、脱落酸和油菜素类固醇的生物合成和/或信号传导。植物经常暴露于高水平的一氧化氮（NO），影响发育和应激触发的反应。然而，植物感知NO的方式在很大程度上仍然是未知的。在这里，我们结合联合收割机的早期变化的分析，暴露于短的急性脉冲的外源NO与识别的转录因子（TF）参与NO传感的植物的转录组。NO响应转录组富含激素稳态和信号相关基因。为了评估参与下胚轴中NO传感的事件，我们使用了基于在黄化幼苗中NO诱导的下胚轴伸长抑制的功能传感测定。激素相关的突变体和有条件表达拟南芥TF的转基因株系的TRANSPLANTA集合被筛选用于NO触发的下胚轴缩短。这些方法允许鉴定与烟草相关的TF、乙烯感知和信号传导、独脚金内酯生物合成和信号传导以及水杨酸盐的产生和积累，这些对于下胚轴NO感测是必需的或调节下胚轴NO感测。此外，NO抑制下胚轴伸长通过一些脱落酸（阿坝）受体和转录编码油菜素类固醇信号成分的正和负调节，从而也牵连这些激素在NO传感。
An NO-sensing mechanism controlling hypocotyl growth in etiolated seedlings requires the biosynthesis and/or signaling of ethylene, strigolactones, salicylate, abscisic acid, and brassinosteroids. Plants are often exposed to high levels of nitric oxide (NO) that affects development and stress-triggered responses. However, the way in which plants sense NO is still largely unknown. Here we combine the analysis of early changes in the transcriptome of plants exposed to a short acute pulse of exogenous NO with the identification of transcription factors (TFs) involved in NO sensing. The NO-responsive transcriptome was enriched in hormone homeostasis- and signaling-related genes. To assess events involved in NO sensing in hypocotyls, we used a functional sensing assay based on the NO-induced inhibition of hypocotyl elongation in etiolated seedlings. Hormone-related mutants and the TRANSPLANTA collection of transgenic lines conditionally expressing Arabidopsis TFs were screened for NO-triggered hypocotyl shortening. These approaches allowed the identification of hormone-related TFs, ethylene perception and signaling, strigolactone biosynthesis and signaling, and salicylate production and accumulation that are essential for or modulate hypocotyl NO sensing. Moreover, NO inhibits hypocotyl elongation through the positive and negative regulation of some abscisic acid (ABA) receptors and transcripts encoding brassinosteroid signaling components thereby also implicating these hormones in NO sensing.
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影响因子：
5.6
作者：
Arc E;Galland M;Godin B;Cueff G;Rajjou L
通讯作者：
Rajjou L
影响因子：
56.9
作者：
DELANEY, TP;UKNES, S;RYALS, J
通讯作者：
RYALS, J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.9
作者：
Arasimowicz-Jelonek M;Floryszak-Wieczorek J
通讯作者：
Floryszak-Wieczorek J
影响因子：
2.9
作者：
Bharti, Niharika;Bhatla, Satish C.
通讯作者：
Bhatla, Satish C.