Adenosine Methylation in Arabidopsis mRNA is Associated with the 3' End and Reduced Levels Cause Developmental Defects.
Adenosine Methylation in Arabidopsis mRNA is Associated with the 3' End and Reduced Levels Cause Developmental Defects.
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DOI：
10.3389/fpls.2012.00048
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发表时间：
2012
影响因子：
5.6
通讯作者：
Fray RG
中科院分区：
文献类型：
--
作者：
Bodi Z;Zhong S;Mehra S;Song J;Graham N;Li H;May S;Fray RG
我们先前表明，N6 - 甲基腺苷（m6A）mRNA甲基化酶在拟南芥胚胎发育过程中是必不可少的。我们还证明这种修饰在所有成熟组织中以不同水平存在。然而，成熟植株对m6A的需求尚未经过测试。在此我们表明，在后期生长阶段m6A水平降低90%会导致植株生长模式改变且顶端优势减弱。这些植株的花朵通常在花器官数量、大小和特性方面存在缺陷。对m6A水平降低的植株的基因表达进行全局分析表明，大量下调基因参与运输或靶向运输，而大多数上调基因参与应激和刺激响应过程。对断裂mRNA中m6A分布的分析表明，m6A主要位于转录本的3′端，在多聚（A）尾之前100 - 150bp的区域。除了分析低甲基化拟南芥植株的表型变化外，我们还将综述mRNA内部甲基化领域的最新进展。
We previously showed that the N6-methyladenosine (m6A) mRNA methylase is essential during Arabidopsis thaliana embryonic development. We also demonstrated that this modification is present at varying levels in all mature tissues. However, the requirement for the m6A in the mature plant was not tested. Here we show that a 90% reduction in m6A levels during later growth stages gives rise to plants with altered growth patterns and reduced apical dominance. The flowers of these plants commonly show defects in their floral organ number, size, and identity. The global analysis of gene expression from reduced m6A plants show that a significant number of down-regulated genes are involved in transport, or targeted transport, and most of the up-regulated genes are involved in stress and stimulus response processes. An analysis of m6A distribution in fragmented mRNA suggests that the m6A is predominantly positioned toward the 3′ end of transcripts in a region 100–150 bp before the poly(A) tail. In addition to the analysis of the phenotypic changes in the low methylation Arabidopsis plants we will review the latest advances in the field of mRNA internal methylation
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影响因子：
14.9
作者：
Clancy, MJ;Shambaugh, ME;Bokar, JA
通讯作者：
Bokar, JA
影响因子：
5.4
作者：
ALONI, Y;DHAR, R;KHOURY, G
通讯作者：
KHOURY, G
影响因子：
64.5
作者：
HULSKAMP, M;MISERA, S;JURGENS, G
通讯作者：
JURGENS, G
DOI：
10.1016/0304-4211(81)90071-7
发表时间：
1981-01-01
期刊：
PLANT SCIENCE LETTERS
影响因子：
--
作者：
NICHOLS, JL;WELDER, L
通讯作者：
WELDER, L
DOI：
10.1139/o79-112
发表时间：
1979-01-01
期刊：
CANADIAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KENNEDY, TD;LANE, BG
通讯作者：
LANE, BG