Albumin: a Galpha(s)-specific guanine nucleotide dissociation inhibitor and GTPase activating protein.
Albumin: a Galpha(s)-specific guanine nucleotide dissociation inhibitor and GTPase activating protein.
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白蛋白：一种 Galpha(s) 特异性鸟嘌呤核苷酸解离抑制剂和 GTP 酶激活蛋白。
DOI：
10.1016/s0003-9861(03)00263-7
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发表时间：
2003
影响因子：
3.9
通讯作者：
Patel,TarunB
中科院分区：
文献类型：
--
作者：
Du,Ziyun;Patel,TarunB
Heterotrimeric GTP binding protein (G protein)-mediated signal transduction events are regulated by their effectors and regulators of G protein signaling (RGS) protein family. The latter proteins function as GTPase activating proteins (GAPs) for G protein α subunits and terminate signaling events. In a search for proteins that modulate the activity of the stimulatory G protein of adenylyl cyclase (Gαs), we found that bovine serum albumin (BSA) inhibits the steady-state GTPase activity of Gαs, but not the inhibitory G protein (Gαi1). This effect of BSA is mediated by decreasing the rate of GDP dissociation from Gαsand decreasing the rate of GTP binding. Thus, BSA functions as a guanine nucleotide dissociation inhibitor for Gαs. Moreover, BSA also increased the intrinsic GTPase activity of Gαs, but not Gαi1, demonstrating that BSA functions as a Gαs-specific GAP. Using mutants of Gαs(Q227L, Q227N, R201C, and R201K), we demonstrate that BSA mediates its GAP function by modulating the ability of R201 to increase GTPase activity. Moreover, using wild-type and Q227N forms of Gαs, our studies demonstrate that the GDI function of BSA decreases the ability of Gαsto stimulate adenylyl cyclase. These findings assign a novel function to BSA as a regulator of G protein signaling.
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影响因子：
56.9
作者：
Sunahara, RK;Tesmer, JJG;Sprang, SR
通讯作者：
Sprang, SR
腺苷酸环化酶促进信号的起始和终止。
DOI：
10.1126/science.283.5406.1328
发表时间：
1999
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Scholich,K;Mullenix,JB;Wittpoth,C;Poppleton,HM;Pierre,SC;Lindorfer,MA;Garrison,JC;Patel,TB
通讯作者：
Patel,TB
表皮生长因子受体胞质结构域中的一个区域相反地调节腺苷酸环化酶的刺激性和抑制性鸟嘌呤核苷酸结合调节蛋白。
DOI：
10.1073/pnas.92.6.2229
发表时间：
1995
影响因子：
11.1
作者：
Sun,H;Seyer,JM;Patel,TB
通讯作者：
Patel,TB
DOI：
10.1073/pnas.94.7.2915
发表时间：
1997-04
影响因子：
11.1
作者：
K. Scholich;A. Barbier;J. Mullenix;T. Patel
通讯作者：
K. Scholich;A. Barbier;J. Mullenix;T. Patel
腺苷酸环化酶上的区域对于抑制异源三聚体 G 蛋白的 βγ 和 Giα 亚基的活性是必需的
DOI：
--
发表时间：
1999
期刊：
影响因子：
--
作者：
C. Wittpoth;K. Scholich;Y. Yigzaw;T. Stringfield;T. Patel
通讯作者：
T. Patel