Widespread over-expression of the X chromosome in sterile F₁hybrid mice.
Widespread over-expression of the X chromosome in sterile F₁hybrid mice.
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DOI：
10.1371/journal.pgen.1001148
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发表时间：
2010-09-30
期刊：
影响因子：
4.5
通讯作者：
Nachman MW
中科院分区：
文献类型：
--
作者：
Good JM;Giger T;Dean MD;Nachman MW
X染色体经常在物种间杂交雄性不育中发挥核心作用，但目前尚不清楚这是否反映了潜在的调控不亲和性。在这里，我们将表型数据与全基因组表达数据相结合，直接将异常表达模式与两种小鼠之间的杂交雄性不育联系起来。我们使用了一个反向杂交，其中F1雄性在一个方向上是不育的，在另一个方向上是可育的，这使得我们能够将表达差异与不育联系在一起，而不是与其他杂交表型联系起来。我们发现了X染色体在不育雄性精子发生过程中广泛过度表达的证据，但在可生育的F1杂交雄性中没有。在减数分裂后正常表达的基因中，过度表达最为明显，这与精子发生后期X染色体范围内的表达中断一致。这种模式不是X染色体更快进化分化的简单结果，因为X连锁表达在两个物种之间高度保守。因此，X染色体在精子发生过程中的转录调控似乎对物种之间的进化差异特别敏感。总体而言，这些数据为家鼠生殖隔离提供了潜在的调控基础，并强调了X染色体转录调控对杂交雄性不育进化的重要性。X染色体在物种间生殖隔离的发展中起着重要作用，但其基础尚不清楚。一种可能的解释是，精子发育对X连锁基因调控的破坏很敏感。在小鼠身上，到目前为止，还缺乏将X染色体上的异常基因表达与生殖隔离联系起来的证据。在这里，我们使用小鼠种内和种间的实验杂交以及全基因组的表达数据来识别与杂交雄性不育相关的异常表达模式。我们观察到在不育的杂交种雄性精子发生过程中，X染色体在整个染色体范围内过度表达，并在发育过程中定位于X染色体失活的明显破坏。综上所述，这些结果强调了基因调控对生殖隔离进化的重要性，并支持了X染色体在精子发生过程中的不适当表达是导致杂交雄性不育快速进化的重要机制的假说。
The X chromosome often plays a central role in hybrid male sterility between species, but it is unclear if this reflects underlying regulatory incompatibilities. Here we combine phenotypic data with genome-wide expression data to directly associate aberrant expression patterns with hybrid male sterility between two species of mice. We used a reciprocal cross in which F1 males are sterile in one direction and fertile in the other direction, allowing us to associate expression differences with sterility rather than with other hybrid phenotypes. We found evidence of extensive over-expression of the X chromosome during spermatogenesis in sterile but not in fertile F1 hybrid males. Over-expression was most pronounced in genes that are normally expressed after meiosis, consistent with an X chromosome-wide disruption of expression during the later stages of spermatogenesis. This pattern was not a simple consequence of faster evolutionary divergence on the X chromosome, because X-linked expression was highly conserved between the two species. Thus, transcriptional regulation of the X chromosome during spermatogenesis appears particularly sensitive to evolutionary divergence between species. Overall, these data provide evidence for an underlying regulatory basis to reproductive isolation in house mice and underscore the importance of transcriptional regulation of the X chromosome to the evolution of hybrid male sterility. The X chromosome plays an important role in the development of reproductive isolation between species, but the basis for this has remained unclear. One possible explanation is that sperm development is sensitive to disruption of X-linked gene regulation. In mice, evidence linking abnormal gene expression on the X chromosome with reproductive isolation has been lacking until now. Here we use experimental crosses within and between species of mice and genome-wide expression data to identify aberrant expression patterns associated with hybrid male sterility. We observed chromosome-wide over-expression of the X chromosome during spermatogenesis in sterile hybrid males and developmentally localized this breakdown to an apparent disruption of X-inactivation. Collectively, these results highlight the importance of gene regulation to the evolution of reproductive isolation and support the hypothesis that improper expression of the X chromosome during spermatogenesis is an important mechanism contributing to the rapid evolution of hybrid male sterility.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
10.7
作者：
Dean, Matthem D.;Good, Jeffrey M.;Nachman, Michael W.
通讯作者：
Nachman, Michael W.
种群基因组学：果蝇果蝇中多态性和差异的全基因组分析。
影响因子：
9.8
作者：
Begun, David J.;Holloway, Alisha K.;Stevens, Kristian;Hillier, LaDeana W.;Poh, Yu-Ping;Hahn, Matthew W.;Nista, Phillip M.;Jones, Corbin D.;Kern, Andrew D.;Dewey, Colin N.;Pachter, Lior;Myers, Eugene;Langley, Charles H.
通讯作者：
Langley, Charles H.
影响因子：
64.8
作者：
Bustamante, CD;Fledel-Alon, A;Clark, AG
通讯作者：
Clark, AG
影响因子：
3.3
作者：
FRANK, SA
通讯作者：
FRANK, SA