Phylogenomic Analysis of Cytochrome P450 Gene Superfamily and Their Association with Flavonoids Biosynthesis in Peanut (Arachis hypogaea L.).
Phylogenomic Analysis of Cytochrome P450 Gene Superfamily and Their Association with Flavonoids Biosynthesis in Peanut (Arachis hypogaea L.).
复制标题
DOI：
10.3390/genes14101944
复制
发表时间：
2023-10-15
期刊：
影响因子：
3.5
通讯作者：
Yang, Xiangli
中科院分区：
文献类型：
--
作者：
Zhang, Kun;Qin, Yongmei;Sun, Wei;Shi, Hourui;Zhao, Shuzhen;He, Liangqiong;Li, Changsheng;Zhao, Jin;Pan, Jiaowen;Wang, Guanghao;Han, Zhuqiang;Zhao, Chuanzhi;Yang, Xiangli
细胞色素P450是植物中广泛存在的酶超家族，在植物生长发育所必需的多种生物合成和解毒途径中发挥着关键作用，如类黄酮生物合成途径。然而，在花生这一全球重要的经济作物中，尚未对其进行系统的研究。这项研究通过全面的全基因组分析解决了这一知识缺陷，导致在花生中鉴定出589个AhCYP基因。通过系统发育分析，所有的AhCyp被系统地划分为9个家族，43个基因家族。基因家族成员数量的变化表明生物功能的专门化。有趣的是，串联复制和片段复制事件都已成为AhCYP超家族进化扩张的关键驱动因素。KA/KS分析强调了强净化选择对AhCyp进化的实质性影响。此外，我们选择了21个基因，编码8个与类黄酮途径相关的酶。实时定量聚合酶链式反应(qRT-PCR)的结果揭示了花生种皮发育过程中的阶段特异性表达模式，粉红色和红色种皮之间存在明显的差异。重要的是，我们确定了基因表达水平和代谢物积累之间的直接关联。这些发现为阐明AHCYP的综合功能和调控不同颜色种皮中黄酮类化合物差异积累的潜在机制提供了有价值的见解。
Cytochrome P450s (CYPs) constitute extensive enzyme superfamilies in the plants, playing pivotal roles in a multitude of biosynthetic and detoxification pathways essential for growth and development, such as the flavonoid biosynthesis pathway. However, CYPs have not yet been systematically studied in the cultivated peanuts (Arachis hypogaea L.), a globally significant cash crop. This study addresses this knowledge deficit through a comprehensive genome-wide analysis, leading to the identification of 589 AhCYP genes in peanuts. Through phylogenetic analysis, all AhCYPs were systematically classified into 9 clans, 43 gene families. The variability in the number of gene family members suggests specialization in biological functions. Intriguingly, both tandem duplication and fragment duplication events have emerged as pivotal drivers in the evolutionary expansion of the AhCYP superfamily. Ka/Ks analysis underscored the substantial influence of strong purifying selection on the evolution of AhCYPs. Furthermore, we selected 21 genes encoding 8 enzymes associated with the flavonoid pathway. The results of quantitative real-time PCR (qRT-PCR) experiments unveiled stage-specific expression patterns during the development of peanut testa, with discernible variations between pink and red testa. Importantly, we identified a direct correlation between gene expression levels and the accumulation of metabolites. These findings offer valuable insights into elucidating the comprehensive functions of AhCYPs and the underlying mechanisms governing the divergent accumulation of flavonoids in testa of different colors.
登录
查看更多内容
影响因子：
5.3
作者：
Ahmad, Naveed;Zhang, Kun;Ma, Jing;Yuan, Mei;Zhao, Shuzhen;Wang, Mingqing;Deng, Li;Ren, Li;Gangurde, Sunil S.;Pan, Jiaowen;Ma, Changle;Li, Changsheng;Guo, Baozhu;Wang, Xingjun;Li, Aiqin;Zhao, Chuanzhi
通讯作者：
Zhao, Chuanzhi
影响因子：
3.9
作者：
Hathorn, Chellani S.;Sanders, Timothy H.
通讯作者：
Sanders, Timothy H.
影响因子：
7.2
作者：
Clausen, Mette;Kannangara, Rubini M.;Moller, Birger Lindberg
通讯作者：
Moller, Birger Lindberg
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
3.9
作者：
Christman, Lindsey M.;Dean, Lisa L.;Weissburg, Jacy R.
通讯作者：
Weissburg, Jacy R.