Transcriptional networks orchestrating red and pink testa color in peanut.
Transcriptional networks orchestrating red and pink testa color in peanut.
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DOI：
10.1186/s12870-023-04041-0
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发表时间：
2023-01-19
影响因子：
5.3
通讯作者：
Zhao, Chuanzhi
中科院分区：
文献类型：
--
作者：
Ahmad, Naveed;Zhang, Kun;Ma, Jing;Yuan, Mei;Zhao, Shuzhen;Wang, Mingqing;Deng, Li;Ren, Li;Gangurde, Sunil S.;Pan, Jiaowen;Ma, Changle;Li, Changsheng;Guo, Baozhu;Wang, Xingjun;Li, Aiqin;Zhao, Chuanzhi
花生色是花生的一项重要性状，与花生的营养价值和商业价值密切相关。粉红色和红色是花生皮的主要颜色。然而，花生种皮颜色调控的遗传机制尚不完全清楚。为了阐明花生testa调控模式，我们采用bulk RNA-seq方法比较了粉色品种（Y9102）、红色品种（ZH12）以及由Y9102 × ZH12 F4系构建的两个RNA池（散装红色和散装粉色）的样品。共鉴定出2992个差异表达基因（DEGs），其中在红色-粉红色大RNA池和y9102 - zh12中分别上调317个和1334个，下调225个和1116个。KEGG分析表明，这些基因被划分为显著富集的代谢途径，包括苯丙素、类黄酮/花青素、类异黄酮和木质素生物合成途径。值得注意的是，花青素上游调控基因PAL、CHS和CHI在粉色和红色花生中表达上调，表明它们的调控可能发生在睾丸色素沉着出现之前。然而，F3H、DFR、ANS等下游调控基因的差异表达表明，皮色加深不仅取决于它们的基因表达偏倚，还与FLS抑制有关。此外，HCT、IFS、HID、7-IOMT和I2'H基因的下调为通过扰动木质素和异黄酮途径促进花青素积累提供了另一种机制。此外，MYB、bHLH和WRKY转录因子的共表达模块也表明了一个令人着迷的转录激活复合物，其中MYB-bHLH可以通过增加花生花青素的生物合成来利用WRKY作为共同选择。这些发现揭示了操纵花青素生物合成的候选功能基因和潜在策略，以改善花生品种的理想果色。在线版本包含补充材料，可在10.1186/s12870-023-04041-0获得。
Testa color is an important trait of peanut (Arachis hypogaea L.) which is closely related with the nutritional and commercial value. Pink and red are main color of peanut testa. However, the genetic mechanism of testa color regulation in peanut is not fully understood. To elucidate a clear picture of peanut testa regulatory model, samples of pink cultivar (Y9102), red cultivar (ZH12), and two RNA pools (bulk red and bulk pink) constructed from F4 lines of Y9102 x ZH12 were compared through a bulk RNA-seq approach. A total of 2992 differential expressed genes (DEGs) were identified among which 317 and 1334 were up-regulated and 225 and 1116 were down-regulated in the bulk red-vs-bulk pink RNA pools and Y9102-vs-ZH12, respectively. KEGG analysis indicates that these genes were divided into significantly enriched metabolic pathways including phenylpropanoid, flavonoid/anthocyanin, isoflavonoid and lignin biosynthetic pathways. Notably, the expression of the anthocyanin upstream regulatory genes PAL, CHS, and CHI was upregulated in pink and red testa peanuts, indicating that their regulation may occur before to the advent of testa pigmentation. However, the differential expression of down-stream regulatory genes including F3H, DFR, and ANS revealed that deepening of testa color not only depends on their gene expression bias, but also linked with FLS inhibition. In addition, the down-regulation of HCT, IFS, HID, 7-IOMT, and I2’H genes provided an alternative mechanism for promoting anthocyanin accumulation via perturbation of lignin and isoflavone pathways. Furthermore, the co-expression module of MYB, bHLH, and WRKY transcription factors also suggested a fascinating transcriptional activation complex, where MYB-bHLH could utilize WRKY as a co-option during the testa color regulation by augmenting anthocyanin biosynthesis in peanut. These findings reveal candidate functional genes and potential strategies for the manipulation of anthocyanin biosynthesis to improve peanut varieties with desirable testa color. The online version contains supplementary material available at 10.1186/s12870-023-04041-0.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
北极芥末花颜色多态性由花瓣特异性下调在花青素生物合成途径的阈值下控制。
影响因子：
3.7
作者：
Dick CA;Buenrostro J;Butler T;Carlson ML;Kliebenstein DJ;Whittall JB
通讯作者：
Whittall JB
苹果 MYB 转录因子调节耐冷性和花青素积累并经历 MIEL1 介导的降解
影响因子：
13.8
作者：
An, Jian-Ping;Wang, Xiao-Fei;Hao, Yu-Jin
通讯作者：
Hao, Yu-Jin
影响因子：
6.9
作者：
Han Y;Vimolmangkang S;Soria-Guerra RE;Korban SS
通讯作者：
Korban SS
影响因子：
7.4
作者：
Griesser, Markus;Hoffmann, Thomas;Schwab, Wilfried
通讯作者：
Schwab, Wilfried