CD44 targeting magnetic glyconanoparticles for atherosclerotic plaque imaging.
CD44 targeting magnetic glyconanoparticles for atherosclerotic plaque imaging.
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DOI：
10.1007/s11095-013-1021-8
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发表时间：
2014-06
影响因子：
3.7
通讯作者：
Huang, Xuefei
中科院分区：
文献类型：
--
作者：
El-Dakdouki, Mohammad H.;El-Boubbou, Kheireddine;Kamat, Medha;Huang, Ruiping;Abela, George S.;Kiupel, Matti;Zhu, David C.;Huang, Xuefei
细胞表面粘附分子CD44在动脉粥样硬化斑块的发生和发展中起重要作用。我们的目标是开发能够选择性靶向CD44的纳米颗粒，用于通过磁共振成像非侵入性检测动脉粥样硬化斑块。制备了表面固定有透明质酸的磁性糖纳米颗粒。通过酶联免疫吸附试验、流式细胞术和共聚焦显微镜观察这些纳米颗粒与CD44的体外结合。在动脉粥样硬化兔模型上进行斑块的体内磁共振成像。磁性糖纳米颗粒可以选择性地结合CD44。在体内获得的T2* 加权磁共振图像中，使用非常低剂量的磁性纳米颗粒观察到主动脉壁的显著对比度变化，从而可以检测动脉粥样硬化斑块。此外，可以在探针施用后无显著延迟地进行成像。透明质酸纳米颗粒在斑块成像中的选择性通过几个对照实验建立。磁性纳米粒子表面透明质酸使成像动脉粥样硬化斑块在体内的磁共振成像。所需的低剂量的纳米颗粒，成像没有太多延迟的可能性和高生物相容性是这些纳米颗粒作为斑块成像的造影剂的优点。
The cell surface adhesion molecule CD44 plays important roles in the initiation and development of atherosclerotic plaques. We aim to develop nanoparticles that can selectively target CD44 for the non-invasive detection of atherosclerotic plaques by magnetic resonance imaging. Magnetic glyco-nanoparticles with hyaluronan immobilized on the surface have been prepared. The binding of these nanoparticles with CD44 in vitro was evaluated by enzyme linked immunosorbent assay, flow cytometry and confocal microscopy. In vivo magnetic resonance imaging of plaques was performed on an atherosclerotic rabbit model. The magnetic glyconanoparticles can selectively bind CD44. In T2* weighted magnetic resonance images acquired in vivo, significant contrast changes in aorta walls were observed with a very low dose of the magnetic nanoparticles, allowing the detection of atherosclerotic plaques. Furthermore, imaging could be performed without significant delay after probe administration. The selectivity of hyaluronan nanoparticles in plaque imaging was established by several control experiments. Magnetic nanoparticles bearing surface hyaluronan enabled the imaging of atherosclerotic plaques in vivo by magnetic resonance imaging. The low dose of nanoparticles required, the possibility to image without much delay and the high biocompatibility are the advantages of these nanoparticles as contrast agents for plaque imaging.
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影响因子：
4.9
作者：
Alexis F;Pridgen E;Molnar LK;Farokhzad OC
通讯作者：
Farokhzad OC
CD44+ CD24（ - ）前列腺细胞是早期的癌症祖/干细胞，为预后不良的患者提供了模型。
影响因子：
8.8
作者：
Hurt, E. M.;Kawasaki, B. T.;Klarmann, G. J.;Thomas, S. B.;Farrar, W. L.
通讯作者：
Farrar, W. L.
DOI：
10.1084/jem.183.3.1119
发表时间：
1996-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
DeGrendele HC;Estess P;Picker LJ;Siegelman MH
通讯作者：
Siegelman MH
影响因子：
11.2
作者：
Eliaz, RE;Nir, S;Szoka, FC
通讯作者：
Szoka, FC
影响因子：
16.8
作者：
Banerji, Suneale;Wright, Alan J.;Jackson, David G.
通讯作者：
Jackson, David G.