Knockdown of a JmjC domain-containing gene JMJ524 confers altered gibberellin responses by transcriptional regulation of GRAS protein lacking the DELLA domain genes in tomato.
Knockdown of a JmjC domain-containing gene JMJ524 confers altered gibberellin responses by transcriptional regulation of GRAS protein lacking the DELLA domain genes in tomato.
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番茄中含有 JmjC 结构域的基因 JMJ524 的敲低通过缺乏 DELLA 结构域基因的 GRAS 蛋白的转录调节来改变赤霉素反应
DOI：
10.1093/jxb/eru493
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发表时间：
2015-03
影响因子：
6.9
通讯作者：
Ye Z
中科院分区：
文献类型：
--
作者：
Li J;Yu C;Wu H;Luo Z;Ouyang B;Cui L;Zhang J;Ye Z
含有Jumonji-C（JmjC）结构域的蛋白JMJ 524通过转录调节缺乏DELLA结构域基因SGLD 1的GRAS蛋白而赋予改变的赤霉素反应，在番茄生长和发育中起重要作用。这些结果拓宽了我们对含有JmjC结构域的蛋白质的理解，这些蛋白质不仅控制基因DNA甲基化，而且还参与GA反应。植物整合对独立激素和环境信号的反应以在逆境中生存。特别是，植物激素赤霉素（GA）调节各种发育过程和应激反应。在这项研究中，Jumonji-C（JmjC）结构域的基因JMJ 524在番茄中的特点。JMJ 524响应昼夜节律，并通过GA处理上调。通过RNAi敲除JMJ 524导致GA不敏感的矮化表型，具有皱缩的叶片和缩短的节间。然而，在这些转基因植物中，检测到更高水平的内源GAs。通过RNA-seq进行的全基因组基因表达分析表明，两个DELLA样基因SlGLD 1（“缺乏DELLA结构域的GRAS蛋白”）和SlGLD 2的表达水平在JMJ 524-RNAi转基因植物中增加。然而，只有SlGLD 1在番茄中的过表达导致GA不敏感的矮化表型，表明SlGLD 1作为GA信号转导的阻遏物。这项研究提出，JMJ 524是通过改变GA反应，至少部分地通过调节SlGLD 1茎伸长所需的。
Jumonji-C (JmjC) domain-containing protein JMJ524 plays important roles in tomato growth and development through conferring altered gibberellin responses by transcriptional regulation of a GRAS protein lacking the DELLA domain gene SGLD1. These results broaden our understanding of the JmjC domain-containing proteins, which not only control genic DNA methylation but are also involved in the GA response. Plants integrate responses to independent hormonal and environmental signals to survive adversity. In particular, the phytohormone gibberellin (GA) regulates a variety of developmental processes and stress responses. In this study, the Jumonji-C (JmjC) domain-containing gene JMJ524 was characterized in tomato. JMJ524 responded to circadian rhythms and was upregulated by GA treatment. Knockdown of JMJ524 by RNAi caused a GA-insensitive dwarf phenotype with shrunken leaves and shortened internodes. However, in these transgenic plants, higher levels of endogenous GAs were detected. A genome-wide gene expression analysis by RNA-seq indicated that the expression levels of two DELLA-like genes, SlGLD1 (‘GRAS protein Lacking the DELLA domain’) and SlGLD2, were increased in JMJ524-RNAi transgenic plants. Nevertheless, only the overexpression of SlGLD1 in tomato resulted in a GA-insensitive dwarf phenotype, suggesting that SlGLD1 acts as a repressor of GA signalling. This study proposes that JMJ524 is required for stem elongation by altering GA responses, at least partially by regulating SlGLD1.
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影响因子：
4.8
作者：
Elkins, JM;Hewitson, KS;Schofield, CJ
通讯作者：
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通讯作者：
Wilson DM