Cutaneous burn injury induces neuroinflammation and reactive astrocyte activation in the hippocampus of aged mice.
Cutaneous burn injury induces neuroinflammation and reactive astrocyte activation in the hippocampus of aged mice.
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皮肤烧伤诱导老年小鼠海马神经炎症和反应性星形胶质细胞活化。
DOI：
10.1016/j.exger.2022.111975
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发表时间：
2022-11
影响因子：
3.9
通讯作者：
Kovacs, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Walrath, Travis;McMahan, Rachel H.;Idrovo, Juan-Pablo;Quillinan, Nidia;Kovacs, Elizabeth J.
By 2050, one in six people globally will be 65 or older. Confusion and delirium are significant complications after burn injury, especially in the elderly population. The etiology is still unknown, however complications may be driven by pro-inflammatory activation of astrocytes within the hippocampus (HPC) after burn injury. Reduced levels of phosphorylated cyclic-AMP response binding element (pCREB), caused by elevated systemic pro-inflammatory cytokines, could lead to cognitive decline and memory impairment. To examine the effects of remote injury on neuroinflammation in advanced age, young and aged mice were subjected to a 15% total body surface area scald burn or sham injury, and euthanized after 24 hours. Expression of ccl2 and tnfa were measured by qPCR in the whole brain and HPC. Astrocyte activation was measured by immunofluorescence within the HPC. pCREB was measured by immunohistochemistry in the dentate gyrus. We saw an 80-fold increase in ccl2 and a 30-fold elevation in tnfa after injury in the whole brain of aged mice compared to young groups and aged sham mice (p<0.05 and p<0.05, respectively). Additionally, there was a 30-fold increase in ccl2 within isolated HPC of aged injured mice when compared to sham injured animals (p<0.05). When investigating specific HPC regions, immunofluorescence staining showed a >20% rise in glial fibrillary acidic protein (GFAP) positive astrocytes within the cornu ammonis 3 (CA3) of aged injured mice when compared to all other groups (p<0.05). Lastly, we observed a >20% decrease in pCREB staining by immunohistochemistry in the dentate gyrus of aged mice compared to young regardless of injury (p<0.05). These novel data suggest that remote injury in aged, but not young, mice is associated with neuroinflammation and astrocyte activation within the HPC. These factors, paired with an age related reduction in pCREB, could help explain the increased cognitive decline seen in burn patients of advanced age. To our knowledge, we are the first group to examine the impact of advanced age combined with burn injury on inflammation and astrocyte activation within the brain.
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影响因子：
4.6
作者：
Gomez CR;Karavitis J;Palmer JL;Faunce DE;Ramirez L;Nomellini V;Kovacs EJ
通讯作者：
Kovacs EJ
DOI：
10.1016/j.bbi.2016.01.017
发表时间：
2016-11
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Chesnokova V;Pechnick RN;Wawrowsky K
通讯作者：
Wawrowsky K
影响因子：
3.7
作者：
Pinnock SB;Blake AM;Platt NJ;Herbert J
通讯作者：
Herbert J
影响因子：
11
作者：
Bartolotti N;Bennett DA;Lazarov O
通讯作者：
Lazarov O
影响因子：
4.4
作者：
Choudhry, MA;Plackett, TP;Kovacs, KJ
通讯作者：
Kovacs, KJ