Mouse versus rat: Profound differences in meiotic regulation at the level of the isolated oocyte.
Mouse versus rat: Profound differences in meiotic regulation at the level of the isolated oocyte.
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DOI：
10.1002/mrd.21377
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发表时间：
2011-10
影响因子：
2.5
通讯作者：
Downs, Stephen M.
中科院分区：
文献类型：
--
作者：
Downs, Stephen M.
取未成熟小鼠(20~23日龄)和幼鼠(25~27日龄)分别用5IU和12.5IU马绒毛膜促性腺激素处理44~48h，获得卵丘细胞包被卵母细胞(CEO)、裸卵母细胞(DO)或游离卵泡。次黄嘌呤、DBcAMP、IBMX、米力农和8-Br-cGMP能更有效地维持小鼠卵母细胞的减数分裂停滞。鸟苷环化酶激活剂，心钠素，抑制了两个物种的CEO的成熟，但霉酚酸逆转了IBMX维持的小鼠CEO的减数分裂停滞，而大鼠的CEO几乎没有活性。用卵泡刺激素(FSH)和两性调节素诱导IBMX停滞的小鼠(而不是大鼠)发生生发泡破裂(GVB)，而人绒毛膜促性腺激素(HCG)对这两种动物无效。然而，FSH和两性调节素刺激了两种物种的卵丘扩张。FSH和hCG都是小鼠和大鼠卵泡GVB的有效诱导剂，而两性调节素只对小鼠卵泡有刺激作用。改变培养液或改变大分子补充物对FSH诱导的大鼠卵母细胞成熟无影响。AMP激活的蛋白激酶(AMPK)激活剂AICAR对小鼠CEO和DO的成熟有很强的刺激作用，但对大鼠CEO只有微弱的刺激作用，对大鼠DO无效。AMPK抑制剂，化合物C，更有效地阻止了hCG处理的小鼠卵泡和热处理的小鼠CEO的减数分裂诱导。在两个物种的培养CEO中，两种试剂在极体形成方面产生了相反的结果。未成熟的小鼠卵母细胞在hCG诱导成熟前的生发泡中可检测到活性AMPK，但未成熟的大鼠卵母细胞中未检测到活性AMPK；在大鼠和小鼠卵母细胞中，AMPK活性与GVB后的染色质共定位，但在大鼠卵母细胞中不像小鼠卵母细胞那样出现在纺锤极。最后，培养的小鼠和大鼠CEO对能量底物操作表现出不同的成熟反应。这些数据突出了这两个物种在减数分裂调控方面的显著差异，并表明在小鼠中，在卵丘细胞包裹的卵母细胞水平上有更大的控制潜力。
Cumulus cell-enclosed oocytes (CEO), denuded oocytes (DO) or dissected follicles were obtained 44–48 h after priming immature mice (20–23-days-old) with 5 IU or immature rats (25–27-days-old) with 12.5 IU of equine chorionic gonadotropin, and exposed to a variety of culture conditions. Mouse oocytes were more effectively maintained in meiotic arrest by hypoxanthine, dbcAMP, IBMX, milrinone, and 8-Br-cGMP. The guanylate cyclase activator, atrial natriuretic peptide, suppressed maturation in CEO from both species, but mycophenolic acid reversed IBMX-maintained meiotic arrest in mouse CEO with little activity in rat CEO. IBMX-arrested mouse, but not rat, CEO were induced to undergo germinal vesicle breakdown (GVB) by follicle stimulating hormone (FSH) and amphiregulin, while human chorionic gonadotropin (hCG) was ineffective in both species. Nevertheless, FSH and amphiregulin stimulated cumulus expansion in both species. FSH and hCG were both effective inducers of GVB in cultured mouse and rat follicles while amphiregulin was stimulatory only in mouse follicles. Changing the culture medium or altering macromolecular supplementation had no effect on FSH-induced maturation in rat CEO. The AMP-activated protein kinase (AMPK) activator, AICAR, was a potent stimulator of maturation in mouse CEO and DO, but only marginally stimulatory in rat CEO and ineffective in rat DO. The AMPK inhibitor, compound C, blocked meiotic induction more effectively in hCG-treated mouse follicles and heat-treated mouse CEO. Both agents produced contrasting results on polar body formation in cultured CEO in the two species. Active AMPK was detected in germinal vesicles of immature mouse, but not rat, oocytes prior to hCG-induced maturation in vivo; it colocalized with chromatin after GVB in rat and mouse oocytes, but did not appear at the spindle poles in rat oocytes as it did in mouse oocytes. Finally, cultured mouse and rat CEO displayed disparate maturation responses to energy substrate manipulation. These data highlight significant differences in meiotic regulation between the two species, and demonstrate a greater potential in mice for control at the level of the cumulus cell-enclosed oocyte.
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影响因子：
2.5
作者：
Downs, Stephen M.;Ya, Ru;Davis, Christopher C.
通讯作者：
Davis, Christopher C.
影响因子：
4.1
作者：
DEKEL, N;GALIANI, D;SHERIZLY, I
通讯作者：
SHERIZLY, I
影响因子：
3.6
作者：
LaRosa, Cean;Downs, Stephen M.
通讯作者：
Downs, Stephen M.
影响因子：
--
作者：
DOWNS, SM;DANIEL, SAJ;EPPIG, JJ
通讯作者：
EPPIG, JJ
影响因子：
2.7
作者：
Chen, Jing;Downs, Stephen M.
通讯作者：
Downs, Stephen M.