UvKmt6-mediated H3K27 trimethylation is required for development, pathogenicity, and stress response in Ustilaginoidea virens.
UvKmt6-mediated H3K27 trimethylation is required for development, pathogenicity, and stress response in Ustilaginoidea virens.
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UvKmt6 介导的 H3K27 三甲基化是黑粉菌的发育、致病性和应激反应所必需的
DOI：
10.1080/21505594.2021.2008150
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发表时间：
2021-12
期刊：
影响因子：
5.2
通讯作者：
Kou Y
中科院分区：
文献类型：
--
作者：
Meng S;Liu Z;Shi H;Wu Z;Qiu J;Wen H;Lin F;Tao Z;Luo C;Kou Y
多梳抑制复合物2（PRC 2）负责组蛋白H3（H3 K27 me 3）介导的转录沉默的赖氨酸27的三甲基化。目前，它在水稻稻曲病菌中的生物学作用尚不清楚。在这项研究中，我们分析了推定的PRC 2催化亚基UvKmt 6的功能。结果表明，UvKMT 6基因的破坏导致了U.绿色的此外，UvKmt 6是建立H3 K27 me 3修饰所必需的，其覆盖基因组中的321个基因。UvKMT 6的缺失导致629个基因的转录去抑制，其中140个被H3 K27 me 3修饰占据。与RNA-seq和ChIP-seq分析一致，进一步证实UvKmt 6参与编码效应子的基因和与次级代谢产物产生相关的基因（例如PKS、NRPS和细胞色素P450）的转录抑制。值得注意的是，我们发现UvKmt 6参与氧化，渗透，细胞壁和营养饥饿应激反应相关基因的转录抑制。从基因表达和表型的角度来看，UvKmt 6介导的H3 K27 me 3除了在真菌发育、毒力和次生代谢产物的产生等方面具有相对保守的作用外，还在U.绿色的
Polycomb repressive complex 2 (PRC2) is responsible for the trimethylation of lysine 27 of histone H3 (H3K27me3)-mediated transcriptional silencing. At present, its biological roles in the devastating rice pathogenic fungus Ustilaginoidea virens remain unclear. In this study, we analyzed the function of a putative PRC2 catalytic subunit UvKmt6. The results showed that disruption of UvKMT6 resulted in reduced growth, conidiation and pathogenicity in U. virens. Furthermore, UvKmt6 is essential for establishment of H3K27me3 modification, which covers 321 genes in the genome. Deletion of UvKMT6 led to transcriptional derepression of 629 genes, 140 of which were occupied with H3K27me3 modification. Consistent with RNA-seq and ChIP-seq analysis, UvKmt6 was further confirmed to participate in the transcriptional repression of genes encoding effectors and genes associated with secondary metabolites production, such as PKSs, NRPSs and Cytochrome P450s. Notably, we found that UvKmt6 is involved in transcriptional repression of oxidative, osmotic, cell wall and nutrient starvation stresses response-related genes. From the perspective of gene expression and phenotype, in addition to the relatively conservative role in fungal development, virulence and production of secondary metabolites, we further reported that UvKmt6-mdediated H3K27me3 plays a critical role in the response to various stresses in U. virens.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1073/pnas.1303750110
发表时间：
2013-04-09
影响因子：
11.1
作者：
Jamieson, Kirsty;Rountree, Michael R.;Selker, Eric U.
通讯作者：
Selker, Eric U.
黑粉菌植物细胞死亡诱导分泌蛋白的鉴定和表征。
影响因子：
3.5
作者：
Fang, Anfei;Han, Yanqing;Sun, Wenxian
通讯作者：
Sun, Wenxian
影响因子：
5.3
作者：
O'Carroll, D;Erhardt, S;Jenuwein, T
通讯作者：
Jenuwein, T
影响因子：
4.5
作者：
Moeller, Mareike;Schotanus, Klaas;Stukenbrock, Eva H.
通讯作者：
Stukenbrock, Eva H.