A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons.
A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons.
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DOI：
10.1101/gr.138651.112
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发表时间：
2012-12
期刊：
影响因子：
7
通讯作者：
Archidiacono N
中科院分区：
文献类型：
--
作者：
Capozzi O;Carbone L;Stanyon RR;Marra A;Yang F;Whelan CW;de Jong PJ;Rocchi M;Archidiacono N
小型猿类的染色体重排频率比大多数哺乳动物高出20倍。由于它们的复杂性，这些类人猿染色体进化的完整程度还没有完全记录。然而，以前的工作与阵列绘画，BAC-FISH和选择性测序的四个karyomorphs中的两个已经表明，高分辨率的方法可以精确地定义染色体断点和映射的复杂流程的进化染色体重排。在这里，我们使用这些工具来精确地定义发生在剩下的两个核型，属合趾猴（2n = 50）和Hoolock（2n = 38）的重排。这项研究提供了最全面的见解染色体重排参与改造小猿基因组的进化起源。对人类-类人猿同线断裂点的生物信息学分析揭示了与转座因子和片段重复的关联，为可能促进小型类人猿重排的机制提供了一些见解。在不久的将来，gills基因组序列的比较将提供新的见解，以测试有关染色体进化机制的假设。这里提出的同线性块边界和方向，染色体融合和着丝粒重新定位事件的精确定义将有助于这些人类近亲的基因组序列组装。
Chromosome rearrangements in small apes are up to 20 times more frequent than in most mammals. Because of their complexity, the full extent of chromosome evolution in these hominoids is not yet fully documented. However, previous work with array painting, BAC-FISH, and selective sequencing in two of the four karyomorphs has shown that high-resolution methods can precisely define chromosome breakpoints and map the complex flow of evolutionary chromosome rearrangements. Here we use these tools to precisely define the rearrangements that have occurred in the remaining two karyomorphs, genera Symphalangus (2n = 50) and Hoolock (2n = 38). This research provides the most comprehensive insight into the evolutionary origins of chromosome rearrangements involved in transforming small apes genome. Bioinformatics analyses of the human–gibbon synteny breakpoints revealed association with transposable elements and segmental duplications, providing some insight into the mechanisms that might have promoted rearrangements in small apes. In the near future, the comparison of gibbon genome sequences will provide novel insights to test hypotheses concerning the mechanisms of chromosome evolution. The precise definition of synteny block boundaries and orientation, chromosomal fusions, and centromere repositioning events presented here will facilitate genome sequence assembly for these close relatives of humans.
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DOI：
10.1093/bioinformatics/btq033
发表时间：
2010-03-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Quinlan AR;Hall IM
通讯作者：
Hall IM
影响因子：
1.6
作者：
Carbone, Lucia;D'addabbo, Pietro;Rocchi, Mariano
通讯作者：
Rocchi, Mariano
影响因子：
9.8
作者：
ENCODE Project Consortium
通讯作者：
ENCODE Project Consortium
影响因子：
4.1
作者：
Matsudaira, Kazunari;Ishida, Takafumi
通讯作者：
Ishida, Takafumi
影响因子：
2.8
作者：
KOEHLER, U;ARNOLD, N;STANYON, R
通讯作者：
STANYON, R