Differences in biofilm formation and antimicrobial resistance of Pseudomonas aeruginosa isolated from airways of mechanically ventilated patients and cystic fibrosis patients.
Differences in biofilm formation and antimicrobial resistance of Pseudomonas aeruginosa isolated from airways of mechanically ventilated patients and cystic fibrosis patients.
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DOI：
10.1016/j.ijantimicag.2010.12.017
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发表时间：
2011-04
影响因子：
10.8
通讯作者：
Yang K
中科院分区：
文献类型：
--
作者：
Fricks-Lima J;Hendrickson CM;Allgaier M;Zhuo H;Wiener-Kronish JP;Lynch SV;Yang K
Pseudomonas aeruginosa biofilms exhibit increased antimicrobial resistance compared with planktonic isolates and are implicated in the pathogenesis of both acute and chronic lung infections. Whilst antibiotic choices for both infections are based on planktonic antibiotic susceptibility results, differences in biofilm-forming ability between the two diseases have not previously been explored. The aim of this study was to compare differences in biofilm formation and antibiotic resistance of P. aeruginosa isolated from intubated patients and from patients with chronic pulmonary disease associated with cystic fibrosis (CF). The temporal evolution of antibiotic resistance in clonal P. aeruginosa strains isolated from CF patients during periods of chronic infection and acute pulmonary exacerbation was also evaluated. Biofilm formation and biofilm antibiotic susceptibilities were determined using a modified microtitre plate assay and were compared with antibiotic susceptibility results obtained using traditional planktonic culture. Clonality was confirmed using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) analysis. Pseudomonas aeruginosa isolates collected from intubated patients produced substantially more biofilms compared with CF isolates. There was considerable heterogeneity in biofilm-forming ability among the CF isolates and this was unrelated to pulmonary status. Biofilm antibiotic resistance developed rapidly among clonal CF isolates over time, whilst traditional antibiotic resistance determined using planktonic cultures remained stable. There was a significant positive correlation between imipenem/cilastatin and ceftazidime resistance and biofilm-forming ability. The variability in biofilm-forming ability in P. aeruginosa and the rapid evolution of biofilm resistance may require consideration when choosing antibiotic therapy for newly intubated patients and CF patients.
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影响因子：
5.7
作者：
El-Azizi, Mohamed;Rao, Suma;Khardori, Nancy
通讯作者：
Khardori, Nancy
影响因子：
15.1
作者：
Falagas, Matthew E.;Kasiakou, Sofia K.
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Kasiakou, Sofia K.
影响因子：
4.2
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Deligianni E;Pattison S;Berrar D;Ternan NG;Haylock RW;Moore JE;Elborn SJ;Dooley JS
通讯作者：
Dooley JS
影响因子：
14.2
作者：
Korbila, I. P.;Michalopoulos, A.;Falagas, M. E.
通讯作者：
Falagas, M. E.
影响因子：
38.9
作者：
Adair, CG;Gorman, SP;Milligan, KR
通讯作者：
Milligan, KR