Pseudomonas aeruginosa cystic fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro.
Pseudomonas aeruginosa cystic fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro.
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DOI：
10.1186/1471-2180-10-38
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发表时间：
2010-02-08
期刊：
影响因子：
4.2
通讯作者：
Dooley JS
中科院分区：
文献类型：
--
作者：
Deligianni E;Pattison S;Berrar D;Ternan NG;Haylock RW;Moore JE;Elborn SJ;Dooley JS
铜绿假单胞菌被认为生长在囊性纤维化(CF)慢性肺部感染的生物膜中。细菌细胞运动是导致铜绿假单胞菌在生物和非生物表面黏附的主要因素之一。在这项研究中，我们使用分子和显微镜方法来确定铜绿假单胞菌Cf分离株是否具有运动性，并将其与体外生物膜形成能力进行统计关联。我们的调查显示，在生物膜的产生、结构和控制方面存在着广泛的多样性。在96株分离株中，49%有游动能力，27%有抽动能力，52%有集群活动能力，47%无活动能力。生物膜形成的微量平板分析表明，生物膜的形成能力从生物膜缺乏的表型到形成很厚的生物膜的表型都有。对单个菌株的运动性和粘附性的比较表明，游动和抽动的存在对生物膜的生物量有积极的影响。然而，至关重要的是，运动性并不是生物膜形成的绝对要求，因为30个非运动性分离物实际上形成了厚厚的生物膜，而3个运动性分离物仅微弱地附着着鞭毛和IV型菌毛。此外，CLSM分析表明，铜绿假单胞菌的生物被膜形成菌株实际上能够捕获非生物被膜形成菌株，因此可以观察到这些“非生物被膜”细胞作为成熟生物膜结构的一部分。在实验室中不产生生物被膜的临床分离株必须具有在患者肺内生存的能力。我们认为在活体内菌株之间存在协同作用，这使得“不形成生物膜”的菌株能够被整合到生物膜中。因此，在体外明显不形成生物膜的菌株有可能参与生物膜介导的肺纤维化的发病机制。
Pseudomonas aeruginosa is considered to grow in a biofilm in cystic fibrosis (CF) chronic lung infections. Bacterial cell motility is one of the main factors that have been connected with P. aeruginosa adherence to both biotic and abiotic surfaces. In this investigation, we employed molecular and microscopic methods to determine the presence or absence of motility in P. aeruginosa CF isolates, and statistically correlated this with their biofilm forming ability in vitro. Our investigations revealed a wide diversity in the production, architecture and control of biofilm formation. Of 96 isolates, 49% possessed swimming motility, 27% twitching and 52% swarming motility, while 47% were non-motile. Microtitre plate assays for biofilm formation showed a range of biofilm formation ability from biofilm deficient phenotypes to those that formed very thick biofilms. A comparison of the motility and adherence properties of individual strains demonstrated that the presence of swimming and twitching motility positively affected biofilm biomass. Crucially, however, motility was not an absolute requirement for biofilm formation, as 30 non-motile isolates actually formed thick biofilms, and three motile isolates that had both flagella and type IV pili attached only weakly. In addition, CLSM analysis showed that biofilm-forming strains of P. aeruginosa were in fact capable of entrapping non-biofilm forming strains, such that these 'non-biofilm forming' cells could be observed as part of the mature biofilm architecture. Clinical isolates that do not produce biofilms in the laboratory must have the ability to survive in the patient lung. We propose that a synergy exists between isolates in vivo, which allows "non biofilm-forming" isolates to be incorporated into the biofilm. Therefore, there is the potential for strains that are apparently non-biofilm forming in vitro to participate in biofilm-mediated pathogenesis in the CF lung.
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影响因子：
5.1
作者：
Barken, Kim B.;Pamp, Suenje J.;Tolker-Nielsen, Tim
通讯作者：
Tolker-Nielsen, Tim
影响因子：
3.2
作者：
Murray, Thomas S.;Kazmierczak, Barbara I.
通讯作者：
Kazmierczak, Barbara I.
DOI：
10.1073/pnas.76.4.1648
发表时间：
1979-01-01
影响因子：
11.1
作者：
FIGURSKI, DH;HELINSKI, DR
通讯作者：
HELINSKI, DR
影响因子：
3.2
作者：
LAWRENCE, JR;KORBER, DR;CALDWELL, DE
通讯作者：
CALDWELL, DE
影响因子：
3.1
作者：
Head, NE;Yu, HW
通讯作者：
Yu, HW