Quantitative tissue oxygen measurement in multiple organs using 19F MRI in a rat model.
Quantitative tissue oxygen measurement in multiple organs using 19F MRI in a rat model.
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DOI：
10.1002/mrm.22968
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发表时间：
2011-12
影响因子：
3.3
通讯作者：
Rollins, Mark D.
中科院分区：
文献类型：
--
作者：
Liu, Siyuan;Shah, Sameer J.;Wilmes, Lisa J.;Feiner, John;Kodibagkar, Vikram D.;Wendland, Michael F.;Mason, Ralph P.;Hylton, Nola;Hopf, Harriet W.;Rollins, Mark D.
单个器官组织氧水平的测量可以提供信息，帮助评估和优化许多领域的医疗干预措施，包括伤口愈合、复苏策略和癌症治疗。回波平面 19F MRI 此前主要关注低氧水平 (pO2) < 30 mmHg 下的肿瘤氧测量。它利用六氟苯 (HFB) 的自旋晶格弛豫率 (R1) 与 pO2 之间的线性关系。在大鼠模型中研究了该技术用于更广泛的 pO2 值和个体器官组织 pO2 测量的可行性。使用 19F 饱和恢复回波平面成像 (EPI) 测量 HFB 的自旋晶格弛豫时间 (T1=1/R1)。初步体外研究验证了 HFB 在 25°C、37°C 和 41°C、7 特斯拉、0 mmHg 至 760 mmHg 氧分压范围内，R1 和 pO2 之间的线性关系。体内实验测量了大鼠吸入 30% 和 100% 氧气时大脑、肾脏、肝脏、肠道、肌肉和皮肤的组织氧 (ptO2) 水平。所有器官的 ptO2 值均随高氧而显着增加 (p<0.001)。这项研究表明，HFB 的 19F MRI 提供了一种测量体内局部 ptO2 的可行工具，并且高氧显着增加了大鼠模型中多个器官的 ptO2。
Measurement of individual organ tissue oxygen levels can provide information to help evaluate and optimize medical interventions in many areas including wound healing, resuscitation strategies, and cancer therapeutics. Echo planar 19F MRI has previously focused on tumor oxygen measurement at low oxygen levels (pO2) < 30 mmHg. It uses the linear relationship between spin-lattice relaxation rate (R1) of hexafluorobenzene (HFB) and pO2. The feasibility of this technique for a wider range of pO2 values and individual organ tissue pO2 measurement was investigated in a rat model. Spin-lattice relaxation times (T1=1/R1) of HFB were measured using 19F saturation recovery echo planar imaging (EPI). Initial in vitro studies validated the linear relationship between R1 and pO2 from 0 mmHg to 760 mmHg oxygen partial pressure at 25°C, 37°C, and 41°C at 7 Tesla for HFB. In vivo experiments measured rat tissue oxygen (ptO2) levels of brain, kidney, liver, gut, muscle and skin during inhalation of both 30% and 100% oxygen. All organ ptO2 values significantly increased with hyperoxia (p<0.001). This study demonstrates that 19F MRI of HFB offers a feasible tool to measure regional ptO2 in vivo, and that hyperoxia significantly increases ptO2 of multiple organs in a rat model.
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影响因子：
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