TaLAMP1 Plays Key Roles in Plant Architecture and Yield Response to Nitrogen Fertilizer in Wheat.
TaLAMP1 Plays Key Roles in Plant Architecture and Yield Response to Nitrogen Fertilizer in Wheat.
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DOI：
10.3389/fpls.2020.598015
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发表时间：
2020
影响因子：
5.6
通讯作者：
Tong Y
中科院分区：
文献类型：
--
作者：
Shi J;Tong Y
了解小麦对氮肥反应的分子机制有助于培育高产高效的小麦品种。在这里，我们克隆了TaLAMP 1 -3A，-3B和-3D，它们在小麦的根和芽中被低氮可用性上调。在水培条件下，TaLAMP 1基因的过表达和敲除均降低了小麦幼苗的侧根长度和氮素吸收量。在正常供氮条件下，过量表达TaLAMP 1 -3B的小麦籽粒产量显著降低（14.5%），敲低TaLAMP 1的小麦籽粒产量显著降低（15.5%）。与野生型（WT）相比，TaLAMP 1 -3B过表达的穗粒数显著增加（7.2%），但穗数显著减少（19.2%）; TaLAMP 1敲除的穗粒数显著减少（15.3%），但穗数与WT相比无差异。结合常氮和低氮条件下的田间试验数据，TaLAMP 1基因的过表达和敲除均抑制了氮肥对水稻产量的影响。过量表达TaLAMP 1 - 3 B显著增加了籽粒氮浓度，在低氮条件下对籽粒产量没有显著的不利影响;因此，TaLAMP 1 -3 B在低投入农业的工程小麦中是有价值的。这些结果表明，TaLAMP 1是关键的小麦适应氮供应和塑造植株结构，通过调节穗数每株和穗粒数。优化TaLAMP 1的表达可以促进小麦育种，提高产量，籽粒氮浓度和对氮肥的产量反应。
Understanding the molecular mechanisms in wheat response to nitrogen (N) fertilizer will help us to breed wheat varieties with improved yield and N use efficiency. Here, we cloned TaLAMP1-3A, -3B, and -3D, which were upregulated in roots and shoots of wheat by low N availability. In a hydroponic culture, lateral root length and N uptake were decreased in both overexpression and knockdown of TaLAMP1 at the seedling stage. In the field experiment with normal N supply, the grain yield of overexpression of TaLAMP1-3B is significantly reduced (14.5%), and the knockdown of TaLAMP1 was significantly reduced (15.5%). The grain number per spike of overexpression of TaLAMP1-3B was significantly increased (7.2%), but the spike number was significantly reduced (19.2%) compared with wild type (WT), although the grain number per spike of knockdown of TaLAMP1 was significantly decreased (15.3%), with no difference in the spike number compared with WT. Combined with the agronomic data from the field experiment of normal N and low N, both overexpression and knockdown of TaLAMP1 inhibited yield response to N fertilizer. Overexpressing TaLAMP1-3B greatly increased grain N concentration with no significant detrimental effect on grain yield under low N conditions; TaLAMP1-3 B is therefore valuable in engineering wheat for low input agriculture. These results suggested that TaLAMP1 is critical for wheat adaptation to N availability and in shaping plant architecture by regulating spike number per plant and grain number per spike. Optimizing TaLAMP1 expression may facilitate wheat breeding with improved yield, grain N concentration, and yield responses to N fertilizer.
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