WRKY45-dependent priming of diterpenoid phytoalexin biosynthesis in rice and the role of cytokinin in triggering the reaction.
WRKY45-dependent priming of diterpenoid phytoalexin biosynthesis in rice and the role of cytokinin in triggering the reaction.
复制标题
DOI：
10.1007/s11103-014-0221-x
复制
发表时间：
2014-09
影响因子：
5.1
通讯作者：
Takatsuji, Hiroshi
中科院分区：
文献类型：
--
作者：
Akagi, Aya;Fukushima, Setsuko;Okada, Kazunori;Jiang, Chang-Jie;Yoshida, Riichiro;Nakayama, Akira;Shimono, Masaki;Sugano, Shoji;Yamane, Hisakazu;Takatsuji, Hiroshi
植物活化剂如苯并噻二唑（BTH）通过引发水杨酸（SA）信号传导途径来保护植物免受疾病。在水稻中，转录因子WRKY45在这一过程中起着重要作用。为了研究BTH引发防御的机制以及WRKY45在这一过程中的作用，我们分析了水稻-稻瘟病菌互作过程中二萜类植物抗毒素（DPs）生物合成基因的转录本。在BTH处理的水稻植株中，DP生物合成基因几乎没有上调，但在M.以WRKY45依赖的方式感染。这些结果表明BTH通过WRKY 45引发了DP生物合成基因。在M.感染WRKY45-过表达（WRKY45-ox）植物。WRKY45-ox和BTH预处理的水稻在M.肺部感染。以前，我们报道了细胞分裂素（CK），特别是异戊烯腺嘌呤，积累在M。受水稻感染的水稻在这里，我们表明，DP生物合成基因的调节SA/CK协同作用的WRKY45依赖的方式。因此，我们认为CK在介导M信号中起作用。BTH引发的植物中DP生物合成基因的诱导。本文的在线版本（doi：10.1007/s11103 - 014 - 0221-x）包含补充材料，可供授权用户使用。
Plant activators such as benzothiadiazole (BTH) protect plants against diseases by priming the salicylic acid (SA) signaling pathway. In rice, the transcription factor WRKY45 plays a central role in this process. To investigate the mechanism involved in defense-priming by BTH and the role of WRKY45 in this process, we analyzed the transcripts of biosynthetic genes for diterpenoid phytoalexins (DPs) during the rice–Magnaporthe oryzae interaction. The DP biosynthetic genes were barely upregulated in BTH-treated rice plants, but were induced rapidly after M. oryzae infection in a WRKY45-dependent manner. These results indicate that the DP biosynthetic genes were primed by BTH through WRKY45. Rapid induction of the DP biosynthetic genes was also observed after M. oryzae infection to WRKY45-overexpressing (WRKY45-ox) plants. The changes in gene transcription resulted in accumulation of DPs in WRKY45-ox and BTH-pretreated rice after M. oryzae infection. Previously, we reported that cytokinins (CKs), especially isopentenyladenines, accumulated in M. oryzae-infected rice. Here, we show that DP biosynthetic genes are regulated by the SA/CK synergism in a WRKY45-dependent manner. Together, we propose that CK plays a role in mediating the signal of M. oryzae infection to trigger the induction of DP biosynthetic genes in BTH-primed plants. The online version of this article (doi:10.1007/s11103-014-0221-x) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
5.3
作者：
Nakayama A;Fukushima S;Goto S;Matsushita A;Shimono M;Sugano S;Jiang CJ;Akagi A;Yamazaki M;Inoue H;Takatsuji H
通讯作者：
Takatsuji H
影响因子：
11.8
作者：
Choi, Jaemyung;Huh, Sung Un;Hwang, Ildoo
通讯作者：
Hwang, Ildoo
影响因子：
3.5
作者：
Hasegawa, Morifumi;Mitsuhara, Ichiro;Ohashi, Yuko
通讯作者：
Ohashi, Yuko
影响因子：
7.4
作者：
Grosskinsky, Dominik K.;Naseem, Muhammad;Roitsch, Thomas
通讯作者：
Roitsch, Thomas
影响因子：
4.8
作者：
Okada, Atsushi;Okada, Kazunori;Yamane, Hisakazu
通讯作者：
Yamane, Hisakazu