Phytoplasma and phytoplasma diseases: a review of recent research.
Phytoplasma and phytoplasma diseases: a review of recent research.
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植原体和植原体疾病：近期研究综述。
DOI：
10.14601/phytopathol_mediterr-3300
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发表时间：
2009
影响因子：
2.4
通讯作者：
B. Duduk
中科院分区：
文献类型：
--
作者：
A. Bertaccini;B. Duduk
过去 40 年来，许多植物黄化病与无壁原核生物病原体——植原体有关。这些病原体不能在无菌培养物中生长，因此它们的研究进展主要通过分子技术来实现。世界范围内已有与植原体存在相关的严重疾病流行的描述。其中包括非洲和加勒比地区的椰子致命黄化、主要葡萄种植区的葡萄树黄化以及影响核果和仁果植物的各种疾病。受植原体感染的植物表现出的症状表明生长调节剂的正常平衡受到严重干扰，并且还出现黄化症状，但症状通常无法诊断。基于 16S rDNA 多态性的研究，现在可以通过分子方法检测和表征感染不同植物物种的植原体。通过研究编码核糖体蛋白 S3、tuf、SecY、amp、imp 和其他基因的基因，也证明了植原体的分子多样性。四种植原体基因组已被完全测序，包括两种‘Candidatus phytoplasma asteris’菌株的基因组和‘Ca. phytoplasma asteris’菌株的基因组。 P. mali’ 和 ‘Ca. P. australiense’。其中三个基因组包含大量重复的 DNA 序列，第四个基因组携带近 100 个基因的多个拷贝。考虑到植原体的基因组异常小，这些重复可能与其跨界栖息地及其致病活性有关。还报告了该领域最新发现的展望。
Numerous yellows-type diseases of plants have been associated with wall-less prokaryote pathogens – phytoplasma over the last 40 years. These pathogens cannot be grown in axenic culture so that advances in their study are mainly achieved by molecular techniques. Severe disease epidemics associated with a phytoplasma presence have been described worldwide. These include coconut lethal yellowing in Africa and the Caribbean, grapevine yellows in major viticultural areas and various diseases affecting stone and pome fruit plants. Phytoplasma-infected plants exhibit symptoms suggesting a profound disturbance in the normal balance of growth regulators and also yellows symptoms, but very often the symptomatology is not diagnostic. Detection and characterization of phytoplasmas infecting different plant species are now possible with molecular methods, based on the study of 16S rDNA polymorphisms. Molecular diversity of phytoplasmas is also demonstrated by studying genes coding the ribosomal proteins S3, tuf, SecY, amp, imp and other genes. Four phytoplasma genomes have been fully sequenced, including those of two ‘Candidatus Phytoplasma asteris’ strains, and those of strains of ‘Ca. P. mali’ and ‘Ca. P. australiense’. Three of these genomes contain large amounts of repeated DNA sequence, and the fourth carries multiple copies of almost 100 genes. Considering that phytoplasmas have unusually small genomes, these repeats might be related to their transkingdom habitat and to their pathogenic activity. An outlook of recent findings in the field is also reported.
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Jung, H-Y.、Miyata, S.、Oshima, K.、Kakizawa, S.、Nishikawa, H.、Kuboyama, T.、Ugaki, M.、Hibi, T.、Namba, S.：“第一个完整的核苷酸序列
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郑 H.-Y.、泽柳 T.、柿泽 S.、西川 H.、宫田 S.、大岛 K.、宇垣 M.、李 J.-T.、日比 T.、
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影响因子：
30.8
作者：
Oshima, K;Kakizawa, S;Namba, S
通讯作者：
Namba, S
影响因子：
2.8
作者：
Kakizawa, S;Oshima, K;Namba, S
通讯作者：
Namba, S
针对一种植原体 SecA 膜蛋白的抗体与系统发育不同植原体的抗体发生反应
DOI：
--
发表时间：
2004
期刊：
Phytopathology 94
影响因子：
--
作者：
Wei;W.et al.
通讯作者：
W.et al.