Evaluation of a high throughput starch analysis optimised for wood.
Evaluation of a high throughput starch analysis optimised for wood.
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DOI：
10.1371/journal.pone.0086645
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ferrini F
中科院分区：
文献类型：
--
作者：
Bellasio C;Fini A;Ferrini F
淀粉是树木最重要的长期储备，因此淀粉的分析是有用的生理信息来源。目前公布的木材淀粉分析方案有几个限制，如长程序和中和步骤。食品和饲料中淀粉分析的高通量标准协议代表了一种有价值的替代方案。然而，它们还没有被优化或测试与木本样品。这些具有特定的化学和结构特征，包括干扰次级代谢物的存在、淀粉的低反应性和低淀粉含量。在这项研究中，用于食品和饲料淀粉分析的标准方法（AOAC标准方法996.11）进行了优化，以提高木材中淀粉分析的精密度和准确度。在消化条件和葡萄糖测定中引入了关键修改。然后，通过430淀粉分析的标准在已知的淀粉含量，基质多糖，和木材收集从三个器官（根，树枝，成熟的木材）的四个物种（针叶和开花植物）的优化协议进行了评估。优化的方案被证明是非常精确和准确的（3%），适用于淀粉含量在40 mg和21 µg之间的样品的高通量常规分析（每天35个样品）。样品可包括针叶和开花植物的木质器官和非木质器官，如叶、果实和根茎。
Starch is the most important long-term reserve in trees, and the analysis of starch is therefore useful source of physiological information. Currently published protocols for wood starch analysis impose several limitations, such as long procedures and a neutralization step. The high-throughput standard protocols for starch analysis in food and feed represent a valuable alternative. However, they have not been optimised or tested with woody samples. These have particular chemical and structural characteristics, including the presence of interfering secondary metabolites, low reactivity of starch, and low starch content. In this study, a standard method for starch analysis used for food and feed (AOAC standard method 996.11) was optimised to improve precision and accuracy for the analysis of starch in wood. Key modifications were introduced in the digestion conditions and in the glucose assay. The optimised protocol was then evaluated through 430 starch analyses of standards at known starch content, matrix polysaccharides, and wood collected from three organs (roots, twigs, mature wood) of four species (coniferous and flowering plants). The optimised protocol proved to be remarkably precise and accurate (3%), suitable for a high throughput routine analysis (35 samples a day) of specimens with a starch content between 40 mg and 21 µg. Samples may include lignified organs of coniferous and flowering plants and non-lignified organs, such as leaves, fruits and rhizomes.
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影响因子：
4
作者：
Barbaroux, C;Bréda, N
通讯作者：
Bréda, N
影响因子：
16.8
作者：
Bond, WJ;Midgley, JJ
通讯作者：
Midgley, JJ
影响因子：
4
作者：
Chantuma, P.;Lacointe, A.;Thaler, P.
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Thaler, P.
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4.1
作者：
BAILEY, RW
通讯作者：
BAILEY, RW
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2.7
作者：
DUNN, JP;POTTER, DA;KIMMERER, TW
通讯作者：
KIMMERER, TW