Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model.
Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model.
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在糖尿病大鼠模型中，源自富含血小板血浆的外泌体通过激活 YAP 促进慢性皮肤伤口的上皮再生
DOI：
10.7150/thno.16803
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发表时间：
2017
期刊：
影响因子：
12.4
通讯作者：
Zhang CQ
中科院分区：
文献类型：
--
作者：
Guo SC;Tao SC;Yin WJ;Qi X;Yuan T;Zhang CQ
慢性伤口已成为经济、社会和公共卫生的负担，需要先进的治疗。富血小板血浆（PRP）已被广泛用于治疗慢性伤口，因为它含有丰富的血小板分泌的生长因子。已证明来源于PRP的外泌体（PRP-Exos）包封来自血小板的主要生长因子。这项研究首次表明这些外泌体可能发挥PRP的功能。PRP-Exos可有效诱导内皮细胞和成纤维细胞的增殖和迁移，以改善慢性伤口中的血管生成和再上皮化。我们调节雅普以验证通过雅普活化对成纤维细胞增殖和迁移的PRP-Exos依赖性作用。在体内，我们在糖尿病大鼠模型中观察了用PRP-Exos治疗的慢性伤口中的皮肤愈合过程。我们提供了PRP对慢性溃疡愈合作用的可能分子机制的证据，并描述了一种来自外泌体的有前途的生长因子资源，而没有物种限制。
Chronic wounds have become an economic, social, and public health burden and need advanced treatment. Platelet-rich plasma (PRP) has been used extensively in treatment of chronic wounds because it contains an abundance of growth factors secreted by platelets. The exosomes derived from PRP (PRP-Exos) have been proven to encapsulate principal growth factors from platelets. This study is the first to show that these exosomes may exert the function of PRP. PRP-Exos can effectively induce proliferation and migration of endothelial cells and fibroblasts to improve angiogenesis and re-epithelialization in chronic wounds. We regulated YAP to verify the PRP-Exos-dependent effect on fibroblast proliferation and migration through YAP activation. In vivo, we observed the cutaneous healing process in chronic wounds treated with PRP-Exos in a diabetic rat model. We provide evidence of the probable molecular mechanisms underlying the PRP effect on healing of chronic ulcers and describe a promising resource of growth factors from exosomes without species restriction.
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DOI：
10.1186/1478-811x-12-26
发表时间：
2014-04-11
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Lopatina T;Bruno S;Tetta C;Kalinina N;Porta M;Camussi G
通讯作者：
Camussi G
影响因子：
5
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DOI：
10.1164/rccm.201103-0516oc
发表时间：
2011-12-01
影响因子：
24.7
作者：
Lo Re, Sandra;Lecocq, Marylene;Huaux, Francois
通讯作者：
Huaux, Francois
影响因子：
15.9
作者：
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通讯作者：
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影响因子：
9.5
作者：
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通讯作者：
Santos, Helder A.