Cyanine fluorophore derivatives with enhanced photostability.

Cyanine fluorophore derivatives with enhanced photostability.
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DOI:
10.1038/nmeth.1774
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发表时间:
2011-11-13
期刊:
影响因子:
48
通讯作者:
Blanchard, Scott C.
Blanchard, Scott C.
中科院分区:
生物学1区
文献类型:
--
作者:
Altman, Roger B.;Terry, Daniel S.;Zhou, Zhou;Zheng, Qinsi;Geggier, Peter;Kolster, Rachel A.;Zhao, Yongfang;Javitch, Jonathan A.;Warren, J. David;Blanchard, Scott C.

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Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
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