Cyanine fluorophore derivatives with enhanced photostability.
Cyanine fluorophore derivatives with enhanced photostability.
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DOI:
10.1038/nmeth.1774
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发表时间:
2011-11-13
期刊:
影响因子:
48
通讯作者:
Blanchard, Scott C.
中科院分区:
文献类型:
--
作者:
Altman, Roger B.;Terry, Daniel S.;Zhou, Zhou;Zheng, Qinsi;Geggier, Peter;Kolster, Rachel A.;Zhao, Yongfang;Javitch, Jonathan A.;Warren, J. David;Blanchard, Scott C.
Fluorescence applications requiring high photostability often depend on the use of solution additives to enhance fluorophore performance. Here we demonstrate that the direct or proximal conjugation of cyclooctatetraene (COT), 4-nitrobenzyl alcohol (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability without otherwise affecting its native spectral characteristics. Such conjugation is a powerful means of improving the robustness of fluorescence-based applications demanding long-lived, nonblinking fluorescence emission.
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