Control region and gastric specific transcription of the rat H+,K+‐ATPase α subunit gene
Control region and gastric specific transcription of the rat H+,K+‐ATPase α subunit gene
复制标题
大鼠 H+,K+-ATPase α 亚基基因的控制区和胃特异性转录
DOI：
10.1016/0014-5793(91)80404-q
复制
发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
M. Futai
中科院分区：
文献类型：
--
作者：
K. Oshiman;K. Motojima;S. Mahmood;A. Shimada;S. Tamura;M. Maeda;M. Futai
克隆了大鼠胃H ~+，K ~+-ATP酶α亚基基因，测定了其5′-上游区的核苷酸序列。与人类基因相应部分的序列比较表明，存在高度保守的区域，这可能是重要的特异性转录的α亚基在胃壁细胞。大鼠酶的氨基末端序列（Met-Gly-Lys-Ala-Glu-）与猪和人酶的氨基末端序列相似。大鼠酶的基因组织也与人类基因相似：内含子1、2和9位于与人类基因完全相同的位置，并且与后者一样，外显子6没有被内含子分开。内含子1和2的序列在大鼠、人和猪中高度保守，但与Na+，K+-ATP酶催化亚基基因的序列完全不同。北方杂交结果表明该基因仅在胃粘膜中转录。
The rat gastric H+,K+-ATPase α subunit gene was cloned and the nucleotide sequence of its 5′-upstream region was determined. Sequence comparison with the corresponding part of the human gene indicated the presence of highly conserved regions which may be important for specific transcription of the α subunit in gastric parietal cells. The amino-terminal sequence (Met-Gly-Lys-Ala-Glu-) of the rat enzyme was similar to those of the pig and human enzymes. The gene organization of the rat enzyme was also similar to that of the human gene: introns 1, 2 and 9 were located in exactly the same positions as those in the human gene, and, as in the latter, exon 6 was not separated by an intron. The sequences of introns 1 and 2 were highly conserved among the rat, human and pig genes, but were entirely different from those of Na+,K+-ATPase catalytic subunit genes. Northern blot hybridization indicated that the gene was transcribed only in gastric mucosa.
DOI：
10.1016/s0021-9258(19)38317-6
发表时间：
1990-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gary E. Shull
通讯作者：
Gary E. Shull
大鼠胃 (HK )-ATP 酶的分子克隆。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shull,GE;Lingrel,JB
通讯作者：
Lingrel,JB
人类 Na,K-ATPase α2 基因的表征和基因内限制性片段长度多态性的鉴定。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shull,MM;Pugh,DG;Lingrel,JB
通讯作者：
Lingrel,JB