Dampened neural activity and abolition of epileptic-like activity in cortical slices by active ingredients of spices.
Dampened neural activity and abolition of epileptic-like activity in cortical slices by active ingredients of spices.
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DOI：
10.1038/srep06825
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发表时间：
2014-10-31
影响因子：
4.6
通讯作者：
Markram H
中科院分区：
文献类型：
--
作者：
Pezzoli M;Elhamdani A;Camacho S;Meystre J;González SM;le Coutre J;Markram H
香料活性成分（AIS）主要通过与TRP通道/受体相互作用来调节外周神经系统中的神经反应。本研究探讨了不同的AIS如何调节S1新皮层第5层锥体神经元的神经反应。测试的AIS是TRPV 1/3、TRPM 8或TRPA 1的激动剂。我们的研究结果表明，辣椒素，丁香酚，薄荷醇，icilin和肉桂醛，但不AITC抑制产生的AP的电压和时间依赖性的方式。在TRPM 8阻断剂（BCTC）存在下和在TRPM 8 KO小鼠上进一步测试TRPM 8配体的这种作用。可观察到的效果仍然存在。最后，测试了所选择的AIS对体外加巴嗪诱导的癫痫发作的影响。结果与上述观察结果一致：除肉桂醛外，相同的AIS能够减少AP爆发的数量和持续时间，并增加引发它们所需的gabazine浓度。总之，我们的数据表明，这些AIS中的一些可以通过TRP独立的途径调节脑中的多巴胺能神经元，无论神经元是否受到细胞内或过度活跃的微电路的刺激。
Active ingredients of spices (AIS) modulate neural response in the peripheral nervous system, mainly through interaction with TRP channel/receptors. The present study explores how different AIS modulate neural response in layer 5 pyramidal neurons of S1 neocortex. The AIS tested are agonists of TRPV1/3, TRPM8 or TRPA1. Our results demonstrate that capsaicin, eugenol, menthol, icilin and cinnamaldehyde, but not AITC dampen the generation of APs in a voltage- and time-dependent manner. This effect was further tested for the TRPM8 ligands in the presence of a TRPM8 blocker (BCTC) and on TRPM8 KO mice. The observable effect was still present. Finally, the influence of the selected AIS was tested on in vitro gabazine-induced seizures. Results coincide with the above observations: except for cinnamaldehyde, the same AIS were able to reduce the number, duration of the AP bursts and increase the concentration of gabazine needed to elicit them. In conclusion, our data suggests that some of these AIS can modulate glutamatergic neurons in the brain through a TRP-independent pathway, regardless of whether the neurons are stimulated intracellularly or by hyperactive microcircuitry.
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DOI：
10.1523/jneurosci.6451-10.2011
发表时间：
2011-03-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cavanaugh DJ;Chesler AT;Jackson AC;Sigal YM;Yamanaka H;Grant R;O'Donnell D;Nicoll RA;Shah NM;Julius D;Basbaum AI
通讯作者：
Basbaum AI
影响因子：
3.3
作者：
Frasnelli, J.;Albrecht, J.;Lundstrom, J. N.
通讯作者：
Lundstrom, J. N.
影响因子：
2.9
作者：
Cho, Jeong Seon;Kim, Tae Hoon;Song, Jin-Ho
通讯作者：
Song, Jin-Ho
影响因子：
5.5
作者：
Karnup, S;Stelzer, A
通讯作者：
Stelzer, A
影响因子：
2.9
作者：
Gebhardt, C;Breustedt, JM;Heinemann, U
通讯作者：
Heinemann, U