Clearly imaging and quantifying the kidney in 3D.
Clearly imaging and quantifying the kidney in 3D.
复制标题
对肾脏进行 3D 清晰成像和量化。
DOI：
10.1016/j.kint.2021.04.042
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发表时间：
2021
影响因子：
19.6
通讯作者：
J. Bertram
中科院分区：
文献类型：
--
作者：
Victor G. Puelles;A. Combes;J. Bertram
几十年来，使用二维切片的肾脏显微解剖测量为我们提供了生理和病理条件下肾脏形态的详细知识。然而，近年来组织清除方法的快速发展，结合新型三维成像模式的发展，为肾脏结构和功能提供了新的见解。这篇综述文章描述了一系列新的见解肾脏的发展和疾病最近获得使用这些新的方法。例如，在发育中的肾脏中，这些方法提供了对输尿管分支形态发生、肾单位祖细胞增殖和定型、输尿管尖端细胞和肾单位祖细胞之间的相互作用以及肾单位分割的建立的新理解。在整个成年小鼠肾脏中，组织清除结合光片显微镜可以成像和量化肾小球总数，这是该领域的重大突破。类似的方法提供了新的见解的结构，肾血管和神经支配，肾小管间质重塑，足细胞损失和肥大，囊肿形成，细胞新月形的演变，和肾小球滤过屏障的结构。随着更多的研究者开发和采用额外的清除和成像技术，可以预期在肾脏生物学和病理学的理解方面会有更多的进展。
For decades, measurements of kidney microanatomy using 2-dimensional sections has provided us with a detailed knowledge of kidney morphology under physiological and pathological conditions. However, the rapid development of tissue clearing methods in recent years, in combination with the development of novel 3-dimensional imaging modalities have provided new insights into kidney structure and function. This review article describes a range of novel insights into kidney development and disease obtained recently using these new methodological approaches. For example, in the developing kidney these approaches have provided new understandings of ureteric branching morphogenesis, nephron progenitor cell proliferation and commitment, interactions between ureteric tip cells and nephron progenitor cells, and the establishment of nephron segmentation. In whole adult mouse kidneys, tissue clearing combined with light sheet microscopy can image and quantify the total number of glomeruli, a major breakthrough in the field. Similar approaches have provided new insights into the structure of the renal vasculature and innervation, tubulointerstitial remodeling, podocyte loss and hypertrophy, cyst formation, the evolution of cellular crescents, and the structure of the glomerular filtration barrier. Many more advances in the understanding of kidney biology and pathology can be expected as additional clearing and imaging techniques are developed and adopted by more investigators.
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影响因子：
13.6
作者：
Klingberg, Anika;Hasenberg, Anja;Gunzer, Matthias
通讯作者：
Gunzer, Matthias
影响因子：
19.6
作者：
Combes AN;Wilson S;Phipson B;Binnie BB;Ju A;Lawlor KT;Cebrian C;Walton SL;Smyth IM;Moritz KM;Kopan R;Oshlack A;Little MH
通讯作者：
Little MH
影响因子：
13.6
作者：
Lindstrom, Nils O.;McMahon, Jill A.;McMahon, Andrew P.
通讯作者：
McMahon, Andrew P.
肾脏的光学清除揭示了钾介导的肾小管重塑
影响因子：
8.8
作者：
Turgay Saritas;Victor G. Puelles;James A. McCormick;Paul Welling;David Ellison
通讯作者：
David Ellison
影响因子：
11.8
作者：
Short, Kieran M.;Combes, Alexander N.;Little, Melissa H.
通讯作者：
Little, Melissa H.