Immunohistochemical study on type II collagen-induced arthritis in DBA/1J mice.
Immunohistochemical study on type II collagen-induced arthritis in DBA/1J mice.
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DBA/1J 小鼠 II 型胶原诱导关节炎的免疫组织化学研究。
DOI：
10.1538/expanim.46.259
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发表时间：
1997
影响因子：
2.4
通讯作者：
K. Doi
中科院分区：
文献类型：
--
作者：
M. Suzuki;K. Uetsuka;M. Suzuki;J. Shinozuka;H. Nakayama;K. Doi
我们对 II 型胶原诱导关节炎 (CIA) 小鼠进行了免疫组织化学检查，重点关注血浆蛋白和细胞外基质材料 (ECM) 分布以及粘附分子表达的变化。第一次免疫后6至20周获取用牛II型胶原免疫的雄性DBA/1J小鼠的肢体关节。 CIA早期，滑膜衬层、关节软骨表面及关节腔内可见纤维蛋白、IgG、血管性血友病因子（vWF）和纤连蛋白沉积。在血管翳形成阶段，血管翳中观察到ICAM-1阳性毛细血管显着增殖和LFA-1阳性中性粒细胞显着浸润。增殖的毛细血管血管翳和基底膜的表面部分对IV型胶原和层粘连蛋白呈强阳性。后期，血管翳侵入并破坏关节软骨和软骨下骨，在血管翳表面以及血管翳与软骨交界处观察到溶菌酶和纤连蛋白均呈强阳性免疫染色。目前，CIA 小鼠血浆蛋白和 ECM 物质分布以及粘附分子表达的免疫组织化学结果在许多方面与类风湿关节炎 (RA) 相似。这表明 CIA 是研究 RA 的有用模型。
We performed immunohistochemical examinations on type II collagen-induced arthritis (CIA) mice, focusing attention on the changes in distribution of plasma proteins and extracellular matrix materials (ECM) and in expression of adhesion molecules. The limb joints of male DBA/1J mice immunized with bovine type II collagen were obtained at 6 to 20 weeks after the first immunization. In the early stage of CIA, deposition of fibrin, IgG, von Willebrand factor (vWF) and fibronectin was detected on the surface of the synovial lining layer and articular cartilage and in the articular cavity. In the stage of pannus formation, prominent proliferation of ICAM-1-positive capillaries and marked infiltration of LFA-1-positive neutrophils were observed in the pannus. The superficial portion of the pannus and basement membranes of proliferated capillaries were strongly positive for type IV collagen and laminin. In the late stage, the pannus invaded and destroyed articular cartilage and subchondral bone, and strongly positive immunostainabilities for both lysozyme and fibronectin were observed on the surface of the pannus and at the junctional portion between the pannus and the cartilage. The present immunohistochemical findings on the distribution of plasma proteins and ECM materials and the expression of adhesion molecules in CIA mice were similar to those in rheumatoid arthritis (RA) in many aspects. This suggests that CIA is a useful model for the investigation of RA.
纤维蛋白原-纤维蛋白与成纤维细胞和巨噬细胞的相互作用。
影响因子：
5.2
作者：
Colvin,RB
通讯作者：
Colvin,RB